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ID
Source
Brief title
Health condition
Ageing, Sarcopenia, Mitochondrial Function
Sponsors and support
Wageningen University and Research
Human and Animal Physiology
Intervention
Outcome measures
Primary outcome
The primary objective of this study is to measure mitochondrial capacity using NIRS in order to verify if NIRS can be used to analyse differences in training status in a normal student population.
Secondary outcome
• To measure mitochondrial capacity in gastrocnemius and the wrist flexor muscles to compare which measurement site correlates best to measurements of whole body oxygen consumption.
• To study the correlation between muscle-recovery to pre-exercise state, expressed as Tc measured using NIRS, and whole body-recovery to pre-exercise state, expressed as EPOC measured using indirect calorimetry.
• To critically asses the use of the PBMCs as a functional biomarker and assess correlations with whole body and skeletal muscle mitochondrial capacity.
• To investigate to which extend physical activity questionnaires can be used to reliably estimate VO2max in young and healthy and generally comparatively fit) males
Study objective
Mitochondrial capacity measured using different, non-invasive techniques can be used to analyse mitochondrial capacity in future studies
Study design
Cross-sectional study design
Intervention
Cross-sectional study design in which subject will be selected on training status.
Zodiac, Building 122, Room E0253
Bart Lagerwaard
De Elst 1
Wageningen 6708 WD
The Netherlands
+31 630599129 (Cell), +31 317 482 643 (Office)
bart.lagerwaard@wur.nl
Zodiac, Building 122, Room E0253
Bart Lagerwaard
De Elst 1
Wageningen 6708 WD
The Netherlands
+31 630599129 (Cell), +31 317 482 643 (Office)
bart.lagerwaard@wur.nl
Inclusion criteria
• 18-28 year old male
• BMI 18.5-25 kg/m2
• VO2max ≤ 47 mL/kg/min or VO2max ≥ 57 mL/kg/min
• Performed a valid VO2max test (In order for the test to be considered valid two out of three of the following conditions should be met: 1) The maximal heart rate is within 10 beats of the predicted maximum (220 – age) 2) A plateau in in VO2 was reached; VO2 fails to increase with 150 mL/min, despite an increase in work load 3) Respiratory exchange ratio (RER) ≥ 1.00 has been achieved) and is has a
Exclusion criteria
• Health concerns regarding respiratory and pulmonary diseases, such as COPD, (exercise induced) asthma and cardiovascular disease.
• (known symptoms of) Metabolic diseases, such as type I or II diabetes,
• Regular smoker (defined as smoking >5 cigarettes per week)
• Haemoglobin concentrations below 8.0 mmol/L
• Recent usage (within four months) of supplements with suggestive training effects, such as creatine phosphate, EPO or anabolic steroids.
• Usage of recreational drugs, such as marihuana, amphetamines and cocaine during the study (starting after first screening day)
• Suffers from (sport) injury that hampers maximal exercise performance
• Blood donation during the course of study
• Current participation in other clinical trials
• Employed or undertaking a thesis or internship at the department of Human and Animal Physiology
Design
Recruitment
Followed up by the following (possibly more current) registration
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL6296 |
| NTR-old | NTR6470 |
| CCMO | NL60823.081.17 |
| OMON | NL-OMON45254 |