To measure markers of several pathways of apoptosis in broncho-alveolar lavage fluid in time during MV in children.
ID
Source
Brief title
Condition
- Respiratory disorders NEC
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Soluble death receptor ligands sFasL (relatively to sFas) and TNF*/TRAIL (by
ELISA)
Granzyme A and B, cleaved cytokeratin 18 (by M30 based ELISA)
Active Angiotensin Coverting Enzyme and Angiotensin II as a parameter of death
receptor apoptosis
Total protein, IgM and Albumin, total cell and differential count.
Secondary outcome
n.a.
Background summary
Apoptosis, a form of programmed cell death, plays a key role in many human
disorders, and is also an essential process during organogenesis, including the
development and maturation of the lung after birth. As a consequence,
disruption of the tight regulation of apoptosis in the lungs of infants and
young children may contribute to both short and long term respiratory
dysfunction. Mechanical ventilation (MV) although frequently life-saving, can
also be deleterious to the respiratory system. The pressure driven oxygen
delivery to the lungs during mechanical ventilation may lead to
ventilator-induced lung injury (VILI). Several mechanisms contribute to VILI,
including inflammation and apoptosis of alveolar epithelial cells. Only a
limited number of in vivo clinical studies have evaluated markers of alveolar
epithelial cell apoptosis in relation to MV in adult patients.
Apoptosis of the alveolar cells can be studied in vivo ventilation in
epithelial lining fluid (ELF) of the alveolar epithelial compartment. In
mechanically ventilated children ELF can only be acquired through in
bronchoalveolar lavage (BAL).
We hypothesize that the role of apoptosis during MV may have different
consequences in children than in adults. Insight in the extent and pathways of
apoptosis during MV in children is highly necessary because it may contribute
to less injurious techniques of MV or even to new therapeutic strategies to
prevent short-term and long-term detrimental consequences of MV in this
vulnerable age group
Study objective
To measure markers of several pathways of apoptosis in broncho-alveolar lavage
fluid in time during MV in children.
Study design
Observational cohort study
Study burden and risks
No complications of any kind are expected from the BAL sample collection
procedures. Patient burden will be minimal
Postbus 22660
1100 DD
Nederland
Postbus 22660
1100 DD
Nederland
Listed location countries
Age
Inclusion criteria
All children younger than 5 years of age who are admitted to the PICU for mechanical ventilation
Exclusion criteria
- PaO2/FiO2 ratio <33.3 kPa (250 mm Hg)
- Platelets < 50*109/l, APTT or PTT > 1.5 normal value
- No permission form parents or care takers
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL13101.018.06 |