The aim of the study is to determine the biological activity of specific IgE antibodies and allergens.
ID
Source
Brief title
Condition
- Allergic conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Primary study parameters:
Titers specific IgE (as can be determined in RAST and CAP) do/do not correlate
with histamin release when homologous allergens are applied.
The biological activity of a purified natural allergen does/does not correlate
with a modified/recombinant allergen.
Secondary outcome
not available
Background summary
Buffy coats (i.e. basophils) are used to determine the biological activity of
specific IgE antibodies and allergens. The histamin release assay is an elegant
method which mimicks the allergic reaction, like it is taking place in the
body, as natural as possible.
Study objective
The aim of the study is to determine the biological activity of specific IgE
antibodies and allergens.
Study design
Healthy donors donate basophils which could be sensitized with IgE of allergic
patients. Measurement of histamin after stimulating these basophils enables us
the characterize the biological activity of IgE antibodies and/or allergen
Study burden and risks
The only risk for the donor is the donation of the buffy coat.
Meibergdreef 9
1105 AZ Amsterdam Zuidoost
NL
Meibergdreef 9
1105 AZ Amsterdam Zuidoost
NL
Listed location countries
Age
Inclusion criteria
Healthy male buffy coat donors without IgE sensitization. Hb levels >8.5 mmol/l are required.
Exclusion criteria
Buffy coat donors whose basophils show 1) a high spontaneous histamin release or 2) no histamin release after application of stimuli (non-responders).
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL13018.018.06 |