Our objective is to study the changes in cytokine levels in dermal interstitial fluid (ISF) obtained by a minimally invasive technique after single and repeated sodium lauryl sulphate (SLS) exposure compared to untreated skin. The study will give…
ID
Source
Brief title
Condition
- Epidermal and dermal conditions
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
To monitor the skin reaction during and after the above mentioned exposures, we
will use transepidermal water loss (TEWL) as an effect parameter for the skin
barrier function and erythema for the extent of skin inflammation.
The ISF samples will be analysed for the cytokines IL-1α, IL-1RA, IL-1β, IL-2,
IL-6, IL-8, IL-10 and TNF-α using ELISA.
Secondary outcome
n.a.
Background summary
Chronic irritant contact dermatitis (ICD) occurs frequently in several
occupations, e.g. hairdressing, nursing and metalworking. The mechanism of the
development of chronic ICD and the factors that predispose individuals to this
skin disease are not completely understood. Screening of individuals at risk is
an important activity in occupational medicine, and such screening must be
based on a knowledge of certain predictive characteristics. Understanding the
role of cytokines in skin irritation might help in the identification of
individual susceptibility factors for chronic ICD.
Study objective
Our objective is to study the changes in cytokine levels in dermal interstitial
fluid (ISF) obtained by a minimally invasive technique after single and
repeated sodium lauryl sulphate (SLS) exposure compared to untreated skin. The
study will give insight in cytokines involved in single and repeated skin
irritation. Understanding the mechanism of repeated irritation is of great
importance for the identification of factors associated with individual
susceptibility to chronic ICD.
Study design
Volunteers will be exposed to SLS at several sites on the volar forearms. On
one arm two single SLS exposures (4 hours) will be performed and on the other
forearm three sites will be repeatedly exposed to SLS over a 3-week period (6
hours a day, 4 days a week). Different SLS concentrations will be used on each
skin site.
The ISF samples are obtained after creating micropores in the stratum corneum
using a laser and subsequently ISF is collected using a vacuum pump. Cytokine
levels will be determined in the ISF samples obtained after single, repeated
and untreated skin.
The investigation will start with a pilot experiment, in which the ISF sampling
technique is tested. For this purpose the number micropores to be created in
the stratum corneum is determined and the duration of ISF sampling which is
needed to obtain sufficient amounts of ISF for the cytokine measurements.
Study burden and risks
n.a.
Meibergdreef 15
1105 AZ Amsterdam
Nederland
Meibergdreef 15
1105 AZ Amsterdam
Nederland
Listed location countries
Age
Inclusion criteria
- age between 18 and 55 years
- Caucasian
Exclusion criteria
- skin diseases
- sunbathing or using a tanning bed 2 months prior to, and during, the investigation
- use of inflammation suppressing medicines (e.g. corticosteroids, NSAIDs) or antibiotics one week prior to, and during the investigation
Design
Recruitment
Medical products/devices used
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL11148.018.06 |