Objective: To determine whether genotype of the promoter polymorphism is associated with alterations of mRNA expression of the M2 receptor
ID
Source
Brief title
Condition
- Gastrointestinal motility and defaecation conditions
- Bladder and bladder neck disorders (excl calculi)
- Bronchial disorders (excl neoplasms)
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Main study parameters/endpoints: Genotype for the polymorphism in the M2
receptor gene promoter will be related to abundance of M2 receptor mRNA in
prostatic tissue; mRNA for housekeeping genes and for other muscarinic receptor
subtypes will be internal controls
Secondary outcome
not applicable
Background summary
Rationale: Polymorphisms have been described in the promoter of the gene
encoding the M2 muscarinic acetylcholine receptor, which were reported to be
associated with increased transcriptional activity in airway cells. Whether
this also applies to the lower urinary tract is unclear.
Group benefit appears possible if the results support the hypothesis that
genotype determines phenotype (receptor expression) with regard to the M2
receptor gene; in this case indication and/or dosage of therapeutics acting on
M2 receptors may eventually be found to be affected by genotype.
Study objective
Objective: To determine whether genotype of the promoter polymorphism is
associated with alterations of mRNA expression of the M2 receptor
Study design
Study design: Cross-sectional, observational study
Study burden and risks
Nature and extent of the burden and risks associated with participation,
benefit and group relatedness: Burden and risk are neglible as only a small
amount of blood (single sample of 2 ml) will be used, obtained on an occasion
where venipuncture is performed for medical reasons. The prostatic tissue to be
used will be tissue removed for therapeutic reasons but not required for
pathological analysis (*discarded tissue*).
Meibergdreef 9
1105 AZ Amsterdam-zuidoost
Nederland
Meibergdreef 9
1105 AZ Amsterdam-zuidoost
Nederland
Listed location countries
Age
Inclusion criteria
Male patients treated in the department of Urology of the Amsterdam Medical Center (AMC) of any age. Any patient that receives any kind of treatment in which prostate tissue is harvested and and not required for pathological analysis.
Exclusion criteria
none
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL17808.018.07 |