Is het possible to determine the presence of disseminated tumor cells in blood in patients with colonic cancer who are operated upon laparoscopiccaly?
ID
Source
Brief title
Condition
- Malignant and unspecified neoplasms gastrointestinal NEC
- Gastrointestinal therapeutic procedures
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The presence of disseminated tumor cells in blood, which means an elevated
signal of CK20 and CEA in the RT-qPCR.
Secondary outcome
none
Background summary
Colon cancer is one of the most common cancer in the Netherlands. The most
important predictor of survival is the presence of lymph node or distant
metastasis.
Still, in 30-45% of the patients with curatively resected colon cancer with no
lymph node involvement, local regional recurrence or distant metastasis will
occur.
A plausible explanation could be the dissemination of tumor cells via blood or
lymphatic system.
A method for detection of disseminated tumor cells is RT-qPCR. With RT-qPCR it
is possible to detect specific genetic markers on tumor cells. In colon
carcinoma, the expression of the genetic markers CK-20 and CEA are often
elevated.
A technique which is used more and more in colonic surgery is the laparoscopy.
The main difference between the conventional operation and the laparoscopic is,
besides its minimal invasive character, the approach to the tumour. During
laparoscopy, first the vessels are ligated, after which the tumour will be
surgically removed. In the conventional operation first the tumour is
approached and resected, after which the vessels are ligated.
Because patients with colonic cancer are more often operated upon
laparoscopically, it is usefull to know if disseminated tumor cells can be
determined in peripheral blood.
Study objective
Is het possible to determine the presence of disseminated tumor cells in blood
in patients with colonic cancer who are operated upon laparoscopiccaly?
Study design
In ten patients with a colonic carcinoma which will undergo a curative
laparoscopic surgical resection, blood samples will be taken from the cephalic
vein and the inferior vena cava. A total of 20ml blood (10ml from each
puncture site) will be taken as soon as these two places can be cannulated.
After resection of the tumour, another 20ml blood will be taken from the same
places.
The blood will be taken to the lab for rt-PCR to determine the expression of
CK20 and CEA.
Study burden and risks
The risk of hemorrhage is equal in laparoscopic and conventional surgery.The
risk of infection is minimal due to sterile conditions on the operating room
and not greater than in any other form of surgery.
Pettelaarseweg 629
5216 BN 's Hertogenbosch
Nederland
Pettelaarseweg 629
5216 BN 's Hertogenbosch
Nederland
Listed location countries
Age
Inclusion criteria
proven colonic carcinoma
no pre-operative suspicion of distant metastasis
older than 18 years
written informed consent given
laparoscopic operation
Exclusion criteria
distant metastasis
irresectable tumour
Conversion to open procedure
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL20637.028.07 |