1) Immunohistochemical analysis of skin biopsies of wounds in clients of the GGD to pinpoint wounds in time2) Analyse new combinations / explore new markers using micro-array analysis of those wounds, to expand the method of analysis of skin wounds…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
huidwonden door uitwendig geweld
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Analysis of immunohistochemistry of skin biopsies as a new method of analysis
the timeframe of the wound.
Secondary outcome
Micro-array analysis for detecting new markers and/or new combination of
markers for determining wounds in time.
Background summary
Medical docters of the GGD have to deal with anaylsis of wounds in routine
daily practive. Until now, the only way in doing so is to describe the
macroscopical aspect of the wound, coinciding with the information provided by
the client.
We recently have developed a method in which we were able to pinpoint
skinwounds in time in biopsies derived from surgical specimen and in wounds
derived ar autopsy, using immunohistochemistry of the wound, combining several
proteins of interest for different timepoints of woundhealing.
We now propose to use this protocol of immunohistochemical analysis on
skinbiopsies derived from clients of the GGD.
Study objective
1) Immunohistochemical analysis of skin biopsies of wounds in clients of the
GGD to pinpoint wounds in time
2) Analyse new combinations / explore new markers using micro-array analysis of
those wounds, to expand the method of analysis of skin wounds.
Study design
1) GGD MD will obtain anamnestic information of the clients visiting the GGD
2) GGD MD will describe the macroscopical analysis of the wound
3) Skin biopsies will be taken of the wound. Part of these wounds will be
analysed uysing (immuno)histochemistry. In a few wounds, micro-array analysis
will be performed to expand putative markers that can be used.
4) Anamnestic information, macroscopical analysis and immunohistochemistry will
be combined for wound determination.
Study burden and risks
Theoretically skin biopsies can cause scars. For this wounds of the face will
be excluded from the protocol.
Theoretically biopsies can be interpreted as painfull, for this cooling of the
wounds will be applied.
De Boelelaan 1117
1007 MB Amsterdam
Nederland
De Boelelaan 1117
1007 MB Amsterdam
Nederland
Listed location countries
Age
Inclusion criteria
Skin wound caused by trauma.
Exclusion criteria
*Wounds in the face since the biopsy may leave a scar
*Patient younger than 18 years
*Burn wound(s)
*Pre-existing inflammatory skin disorders such as psoriasis, SLE and dermatitis.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL19961.029.07 |