The present proposal is designed to examine the effect of carbohydrate and protein co-ingestion on muscle protein synthesis during endurance type exercise activities.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
inspanningsfysiologie/spiergroei
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The difference in muscle protein synthesis rates during endurance exercise
between the carbohydrate and carbohydrate plus protein experiment, as measured
by the incorporation of labeled amino acids in muscle tissue.
Secondary outcome
The difference in whole-body protein synthesis and breakdown rates during
endurance exercise between the carbohydrate and carbohydrate plus protein
experiment, as measured by whole-body amino acid tracer kinetics.
Background summary
Recently, we showed that the intake of carbohydrates and protein before and
during exercise augments muscle protein synthesis during resistance exercise.
It might be speculated that the intermittent character of resistance exercise
allows protein synthesis to be elevated in the recovery phases between sets.
Therefore, the effect of carbohydrates and protein co-ingestion on muscle
protein synthesis during continuous endurence exercise remains to be
determined.
Study objective
The present proposal is designed to examine the effect of carbohydrate and
protein co-ingestion on muscle protein synthesis during endurance type exercise
activities.
Study design
Subjects will be tested on 2 occasions, separated by 2 weeks. In the morning
following an overnight fast, muscle protein synthesis rates will be measured
during a resting period of 2 hours and a subsequent exercise period of 2 hours.
During exercise subjects will ingest beverages containing either carbohydrate
or carbohydrate and protein. Treatments will be performed in a double blind and
randomised order. Stable isotope amino acid tracers will be applied and blood
and muscle samples will be taken to simultaneously assess whole-body and muscle
protein synthesis rates.
Intervention
The test starts with a 2-h resting period, to get a steady state for the amino
acid tracer, followed by the first muscle biopsy. After another 2-h of rest,
the second biopsy is taken, to measure basal protein synthesis rates.
Thereafter, the exercise protocol begins, which consists of 2-h cycling at 55%
Wmax. every 15 minutes during exercise, subjects receive a certain amount of
the test drink. At the end of exercise, a third muscle biopsy is taken. Every
30 minutes in rest and every 15 minutes during exercise, a blood sample is
taken. Stable amino acid isotopes are given by venous infusion during the
entire test period.
Study burden and risks
The risks involved in participating in this experiment are minimal. Insertion
of the catheters in a vein is comparable to a normal blood draw and the only
risk is of a small haematoma. This is the same for the muscle biopsy. The
incision made for obtaining the muscle biopsy (performed by an experienced
physician) will heal completely. The labelled amino acid tracers applied are
not radioactive and are completely safe. The test beverages are made from
normal nutritional ingredients and for this reason do not form any health
risks.
Postbus 616
6200 MD Maastricht
Nederland
Postbus 616
6200 MD Maastricht
Nederland
Listed location countries
Age
Inclusion criteria
healthy men
age: 18-28
endurance trained
body mass index (BMI) < 25
Exclusion criteria
use of medication
BMI > 25
Not physically active
Design
Recruitment
Followed up by the following (possibly more current) registration
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Other (possibly less up-to-date) registrations in this register
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In other registers
| Register | ID |
|---|---|
| CCMO | NL22467.068.08 |