In this study, we will assess whether healthy men with high-normal serum CK display attenuated platelet aggregation, compared to those with low-normal serum CK.
ID
Source
Brief title
Condition
- Coagulopathies and bleeding diatheses (excl thrombocytopenic)
- Embolism and thrombosis
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Main study parameters/endpoints: Difference in ADP induced platelet aggregation
with low vs high serum CK.
Secondary outcome
Differences in study groups between other blood parameters, including platelet
count, aPTT, PT, fibrinogen, spontaneous platelet aggegation, P-selectin,
collagen, arachidon acid and ristocetine aggregation tests (before and after 80
mg aspirin), clopidogrel resistance, ADP/ATP ratio in platelets, creatine,
creatinine
Background summary
Black people are reported to have attenuated platelet aggregation and high
serum creatine kinase (CK) activity. Creatine kinase affects the ADP/ATP ratio
through catalysis of the reaction: ADP + creatine phosphate (CrP) <=> ATP +
creatine. A greater flux through the reaction may lead to lower ADP and affect
platelet aggregation. In a pilot study, we found that adding human CK to
platelet-rich plasma attenuated ADP induced platelet aggregation.
Study objective
In this study, we will assess whether healthy men with high-normal serum CK
display attenuated platelet aggregation, compared to those with low-normal
serum CK.
Study design
Observational study
Study burden and risks
The participants will attend once. General history will be taken, 5 tubes of
blood will be drawn once from the participant*s cubital vein, 3 for estimation
of blood values, and 2 for coagulation tests. Participants will then receive a
single dose of aspirin (80 mg), and after 1 hour, 2 more tubes of blood will be
drawn for coagulation tests; in total 7 tubes, around 70 ml blood. With a low
burden to the participant, the resulting data might help explain clinically
relevant ethnic differences in platelet aggregation.
Meibergdreef 9
1105 AZ Amsterdam
NL
Meibergdreef 9
1105 AZ Amsterdam
NL
Listed location countries
Age
Inclusion criteria
healthy men, 18-50 Y
Exclusion criteria
personal and family history of bleeding or coagulation abnormalities, drug use, use of acetylsalicylic acid or other non-steroidal anti-inflammatory drugs in the past week, current smoking, BMI>/= 30 kg/m2; glucose, lipid spectrum, thyroid, kidney, or liver abnormalities, sickle cell disease.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL31567.018.10 |