To compare the expression of inhibitory receptors on immune competent cells in the airways of patients with allergic rhinitis, patients with infectious rhinitis. A secondary aim is to determine the relationship between inhibitory receptor expression…
ID
Source
Brief title
Condition
- Allergic conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
(1) Expression of inhibitory receptors (LAIR-1, SIRL-1, CD200R, CTLA-4 en
SIRP-α) on leukocytes in peripheral blood and nasopharyngeal washes (2) Changes
in inhibitory receptor expression in nasopharyngeal washes during the course of
disease.
Secondary outcome
-Flow cytometry of will be used to study inhibitory receptor expression during
the course of disease
Background summary
Allergic rhinitis is a common chronic disorder in children and adults. Although
the signs and symptoms may be mild, the economic burden is high. The
pathogenesis is extensively studied, but not yet completely understood. It has
been suggested that an imbalance in immune regulation plays an important role.
The hypothesis of this study is that allergic rhinitis is associated with
decreased expression of inhibitory receptors. Our research group has special
interest in inhibitory receptor leukocyte-associated immunoglobulin-like
receptor 1 (LAIR-1).
Study objective
To compare the expression of inhibitory receptors on immune competent cells in
the airways of patients with allergic rhinitis, patients with infectious
rhinitis. A secondary aim is to determine the relationship between inhibitory
receptor expression and disease severity during the course of disease.
Study design
Observational case-control study
Study burden and risks
Due to the type of study, observational, with invasive diagnostic procedures as
previously described, but no adverse or serious adverse events are to be
expected.
-Venous blood (10 ml) will be drawn from all patients by venapuncture.
-Nasopharyngeal wash is a non-invasive technique where mucus is suctioned from
the nose. The burden for the patient is low. Researchers are experienced with
this technique. No complications have been described.
Discomfort caused by nasal sampling will be monitored in 7 patients with
allergic rhinitis and 7 patients with infectious rhinitis by one independent
research nurse or other researcher in the hospital using an 10-point visual
analogue scale. Discomfort will be scored during every sample collection at 3
timepoints: before collection of the aspirate, during aspiration and 5 minutes
after the procedure.
POB 85090, Kamer E4.133.1
3508 AB Utrecht
NL
POB 85090, Kamer E4.133.1
3508 AB Utrecht
NL
Listed location countries
Age
Inclusion criteria
Patients with allergic rhinitis
-Age 18-35 years
-No severe co-morbidity, other than allergic symptoms
-Proven allergic rhinitis (based on clinical evaluation of a medical doctor or with positive RAST) experiencing at least nasal symptoms
-Suffering from outdoor allergies (hay fever, pollen, weeds, grass) or indoor allergies (mold, dust, dust mite) or pet allergies.
- Good understanding of the Dutch language
- Willingness to receive a member of the investigation team in the house of the participant;Controls
-Healthy individuals 18-35 years with a runny nose
Exclusion criteria
-Any severe comorbidity
-Any other infection during the department visit
-Immunological nasal disease (Churg-Strauss syndrome, Sjögren*s syndrome, systemic lupus erythematosus, sarcoidosis, Wegener*s granulomatosis)
-Use of nasal corticosteroid spray 1 month or less prior during the hospital visit.
-Use of oral corticosteroids 1 month or less prior during the hospital visit.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL37135.041.11 |