To fully characterize skeletal muscle strength and endurance, phenotype and muscle fiber type-specific satellite cell content in frail elderly as compared to those elderly that do not classify as frail according to clinical criteria.
ID
Source
Brief title
Condition
- Other condition
- Muscle disorders
Synonym
Health condition
veroudering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Primary study parameters are skeletal muscle fiber characteristics, : muscle
fiber size and satellite cell content in type I and type II muscle fibers.
Secondary outcome
Secondary endpoints: blood amino acid profiles, blood glucose homeostasis,
skeletal muscle function (hand-grip strenght), muscle strength (leg press),
muscle mass and body composition (DEXA and CT).
Background summary
Frailty must be characterized as a state of decreased physiological reserve and
a clinical syndrome of energy dysregulation, leading to an accumulation of
deficits and eventually overt sarcopenia. It has been shown to be an
independent risk factor for adverse outcomes in elderly and, therefore, has
been recognized as an important focus of research. However, most studies
focused on epidemiology of frailty and criteria of definition, rather than
understanding those parameters that contribute to a disturbed skeletal muscle
protein handling. Healthy elderly (and young) populations have shown the ratio
between skeletal muscle stem cells, so-called satellite cells, and the
corresponding muscle fibers to remain fairly constant throughout life. We
hypothesize this ratio to be disturbed in those elderly that are classified as
frail using the validated scores, which will be main objective
Study objective
To fully characterize skeletal muscle strength and endurance, phenotype and
muscle fiber type-specific satellite cell content in frail elderly as compared
to those elderly that do not classify as frail according to clinical criteria.
Study design
Skeletal muscle mass and function, amino acid levels, satellite cell and
skeletal muscle fiber content will be evaluated in frail elderly and controls.
Participants will undergo a DEXA measurement and a CT scan of the thigh muscles
to evaluate body composition. Muscle strenght and endurance will be tested and
venous blood samples will be drawn to evaluate amino acid profiles. In
addition, a skeletal muscle biopsy of the vastus lateralis muscle will be
obtained.
Study burden and risks
Insertion of the catheters in a vein is comparable to a normal blood drawn with
a risk of a small local hematoma. This is the same for the muscle biopsy. The
incision made for obtaining the muscle biopsy (obtained by an experienced
physician) will heal completely. There is also a small risk for infection and a
temporary neuropathy if a small nerve branch was damaged in the biopsy
procedure. A dull muscle ache might be present for a few days after the
procedure.
We estimate the experiments will take a total of approximately 9 hours in 3
mornings.
Although subjects may not benefit personally from participating in this study,
their contribution to research will be beneficial for patients in the future.
Debeyelaan 25
6202 AZ Maastricht
NL
Debeyelaan 25
6202 AZ Maastricht
NL
Listed location countries
Age
Inclusion criteria
frail male, non-obese (BMI <27 kg*m-2) subjects aged 70-90 years, with no history of participating in any regular exercise training program in the past 5 years, meeting 3 or more of these criteria: 1) unintentional weight loss of 10% or more since age of 60 2) self-reported low usual energy level (3 on a 0-10 scale), feeling unusually tired in the last month, or unusually weak in the last month; 3) low energy expenditure, defined as being in the lowest quintile of energy expenditure measured using a six-item version of the Minnesota Leisure Time Activity Questionnaire; 4) slowness, defined as being in the lowest quintile of walking speed over a 4,57-m distance; 5) weakness, defined as being in the lowest quintile of hand grip strength measured using a handheld dynamometer.
Subjects will be considered non-frail and, as such, eligible for inclusion in the study if 1 or less of the criteria is met (i.e. only non-frail individuals in healthy elderly control group).
Exclusion criteria
Exclusion criteria are: type I and type II diabetes, the use of anticoagulation, female sex, senile dementia, participation in any regular exercise program.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL33499.068.10 |