to investigate functional characteristics of immune cells from MS patients, including migratory capacity, inflammasome activity and Breg cell function and compare these to healthy control subjects 2) to assess possible differences with regard to the…
ID
Source
Brief title
Condition
- Autoimmune disorders
- Central nervous system infections and inflammations
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The main study parameters include the percentage of suppression of T cell
proliferation, the percentage in reduction of either adhesion molecules or
migrating immune cells and the secretion of IL-1*. Differences between MS
patients and between MS patients and healthy controls will be investigated.
Secondary outcome
The effects of MS disease activity, type of disease and MS disease medication
on functional immune cell characteristics
Background summary
Multiple Sclerosis is believed to be an auto-immune disease affecting the
central nervous system. Auto-reactive T helper 1(Th1) and Th17 cells and a
defect in the suppressive capacity of regulatory T cells (Treg) seem to be
involved in disease pathogenesis. This defect in Treg suppressive function
results in dysregulation of the effector cells and could therefore be important
in disease pathogenesis. Next to T cells, also B cell suppressive function
gained more interest during the last years in MS. Next to suppressive function,
also migratory capacity of cells across the blood brain barrier is an important
functional feature. In addition to the adaptive immune system, also the innate
immune cells are involved in MS. One important feature is inflammasome
activity. Therefore, we would like to perform a pilot study to explore
different functional characteristics of immune cells, including Breg function,
T cell migration across an endothelial barrier and inflammasome activity in MS
patients. The results may give rise to more extended studies to confirm the
data obtained in the proposed pilot study and could result in relevant
functional targets for testing the efficacy of new treatment strategies in MS.
Study objective
to investigate functional characteristics of immune cells from MS patients,
including migratory capacity, inflammasome activity and Breg cell function and
compare these to healthy control subjects 2) to assess possible differences
with regard to the functional characteristics of immune cells between different
MS subtypes and between different treatment strategies.
Study design
This is a cross sectional explorative study.
Study burden and risks
The intervention that patients have to endure is a venepuncture. The most
important risk of this the development of a haematoma or a vasovagal reaction.
The risks of this intervention is minimal and of temporary nature.
postbus 616
6200 MD Maastricht
NL
postbus 616
6200 MD Maastricht
NL
Listed location countries
Age
Inclusion criteria
Age between 18 and 65
Caucasian offspring
Exclusion criteria
MS patients: - Non- Caucasian offspring
- Use of immune suppressive medication;Healthy controls: - Non- Caucasian offspring
- Use of immune suppressive or immune modulatory medication
- Having MS or another autoimmune disease
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL38408.096.11 |