The primary objective of this study is to test the virucidal efficacy of two alcohol based hand disinfectants (according prEN 12791) against murine norovirus 1 (MNV1) and human noroviruses (G1.4 and GII.4) on artificially contaminated hands. The…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
het gaat niet om aandoeningen; het gaat om de activiteit van handdesinfectiemiddelen tegen norovirussen, op kunstmatig besmette vingertoppen
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The counts of noroviruses on finger tips, direct after application and after
the use of 'hand rubs' or the washing of hands with soap and water give an
indication if there is a difference between both methods or not.
Secondary outcome
na
Background summary
The most important means of preventing norovirus transmission and infection is
exercising frequent and appropriate hand washing. Alcohol-based hand sanitizers
(>=62% ethanol) may be helpful as an adjunct method of hand hygiene, but should
not replace washing with soap and water.
Evidence for efficacy against norovirus is usually based on studies using
murine norovirus (MNV) as a surrogate. However, MNV and human norovirus (HNV)
exhibit different physiochemical properties and it is unclear whether
inactivation of MNV reflects efficacy against norovirus.
In the study described in this proposal the virucidal activity of two alcohol
based hand disinfectants will be tested on MNV, a surrogate for HNV, and on two
types of HNV. The results will be compared with traditional washing with water
and soap. The hypothesis is that alcohol based hand disinfectants are less
effective in inactivating HNV than the removal of norovirus obtained by proper
hand washing. If this is true, avcices will be formulated so that appropriate
hygiene measures can be taken during outbreaks.
Furthermore, the transmission experiments described in the study will give
information about the transfer of noroviruses from contaminated surfaces and
food products to finger pads and from contaminated finger pads to surfaces and
food products. Results will lead to a better understanding of the way of
transmission after repeated contacts.
Study objective
The primary objective of this study is to test the virucidal efficacy of two
alcohol based hand disinfectants (according prEN 12791) against murine
norovirus 1 (MNV1) and human noroviruses (G1.4 and GII.4) on artificially
contaminated hands. The results obtained will be compared to the effect of hand
washing using soap and rinsing water, followed by drying with disposable towels.
The secondary objective is to quantify the decrease in transmission from
artificially contaminated hands (both before and after treatment with an
alcohol rub) to inanimate surfaces and food products.
Study design
see protocol
Intervention
Hands from test persons, where part of the finger tips are artificially
contaminated with norovirus will be treated with 'hand rubs' (according to the
manufacturer's instructions). Traditional handwashing with soap and water is
used as control.
Study burden and risks
The burden for test persons is not quite high. After artificial contamination
of their finger tips with norovirus they have to perform some actions: pressing
finger tips on surfaces, using hand rubs and washing their hands in a
traditional way with soap and water. Finally, a disinfectian step to inactivate
the viruses.
During the session the investigator and assistant talk with the test person. In
between 'commands'are given as described above.
Bomenweg 2
6703 HD Wageningen
NL
Bomenweg 2
6703 HD Wageningen
NL
Listed location countries
Age
Inclusion criteria
Ten persons, employees and/or students, (no cuts and/or abrasions on fingers and not pregnant) will be randomly selected from the persons working in the WUR buildings located at the Dreijen (Wageningen) that answered the inviting email. Both males and females will be included. A form with questions about dermatological disorders will be used for inclusion/exclusion. The persons mentioned under C8, Wilma Hazeleger and Rijkelt Beumer, will be responsible for inclusion/exclusion. Test persons will be checked about 15 min before start of the experiments on dermatological disorders. A final check just before the start of the experiments (within 5 min) will be done by the researcher (Era Tuladhar).
Exclusion criteria
dermatological disorders: cuts, lesions, infections, eczema, psoriasis, and being pregnant
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL39407.081.12 |