To perform 31P-MRS during and after exercise using a specifically designed probe to determine variations in energy metabolism along the TA muscle in healthy humans subjects.
ID
Source
Brief title
Condition
- Musculoskeletal and connective tissue disorders congenital
- Glucose metabolism disorders (incl diabetes mellitus)
- Musculoskeletal and connective tissue disorders NEC
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Main endpoint is the rate of PCr recovery after exercise for the different
regions of the TA muscle.
Secondary outcome
The utilization of PCr, the buildup of inorganic phosphate, and the effect of
exercise on pH during the muscle contractions are secondary endpoints. All
these parameters can be obtained from the same MR spectrum can therefore be
measured simultaneously.
Background summary
Skeletal muscle consists of basically two different fiber types, that are
different in their metabolic capacities: Type I fibers are slowly contracting,
their oxidative potential is high and they are resistant to fatigue. Type II
fibers are fast contracting, their oxidative potential is low and they fatigue
quickly. Invasive studies performed in rodents suggest that the fiber
distribution varies along the length of many muscles including the tibialis
anterior (TA) muscle. 31P magnetic resonance spectroscopy (MRS) can be used to
noninvasively determine the muscle*s metabolic capacities during and after
exercise. Specifically, the recovery rate of phosphocreatine (PCr) after
exercise can be used to determine in vivo oxidative capacity of the muscle.
Since the rate of PCr recovery is lower in type II compared to type I fibers,
it is hypothesized that this parameter varies along the TA muscle in healthy
male volunteers.
Study objective
To perform 31P-MRS during and after exercise using a specifically designed
probe to determine variations in energy metabolism along the TA muscle in
healthy humans subjects.
Study design
In this study, we are testing the acute effects of muscle contractions within
different regions of the same muscle (tibialis anterior).
Intervention
Muscle contractions inside the MR scanner will be used to disturb the metabolic
steady state of the tibialis anterior. Non-invasive 31P-MRs will be performed
before, during, and after these muscle contractions
Study burden and risks
The proposed study is fully non-invasive. Therefore, burden and risk is
minimal.
However, the maximum duration of the MR experiment is relatively long (1.5h).
However, we now from previous studies that this can relatively easily be
tolerated by healthy subjects.
Geert Grooteplein Zuid 10
Nijmegen 6525 GA
NL
Geert Grooteplein Zuid 10
Nijmegen 6525 GA
NL
Listed location countries
Age
Inclusion criteria
Potential volunteers must meet all of the following inclusion criteria:
-healthy
-male
-young: between 18 to 35 years
-BMI between 19 and 25 kg/m2
-MR compatibility is verified using a questionnnaire
Exclusion criteria
A potential subject who meets any of the following criteria will be excluded from participation in this study:
-Epilepsy or medication against epilepsy
-Subject is not MR compatible (e.g. because of pacemaker, claustrophobia, metal implants in the lower leg that may interfere with MRS, metal splinters in the eye)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL47394.091.14 |