The aim of this study is to analyze the long-term effect of full-mouth tooth extraction on the oral microflora, using the 16S next-generation sequencing technique.
ID
Source
Brief title
Condition
- Bacterial infectious disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Change in relative abundance of bacterial species over time as analysed by 16S
next generation sequencing technique.
Secondary outcome
NA
Background summary
From previous research it is known that elimination of the subgingival
environment by extraction of all natural teeth significantly changes the oral
microflora, including the reduction of the periopathogens Aggregatibacter
actinomycetemcomitans and Porphyromonas gingivalis. However, the long-term
effects of full-mouth tooth extraction on the oral microflora are unknown. It
would be interesting to know these long-term effects because nowadays many
fully edentulous subjects receive dental implants. The presence of certain
periodontal pathogens might pose a risk for dental implants.
Study objective
The aim of this study is to analyze the long-term effect of full-mouth tooth
extraction on the oral microflora, using the 16S next-generation sequencing
technique.
Study design
The present study is a prospective cohort study. Patients that have previously
participated (4-5 years ago) in a study evaluating the short-term effects of
full-mouth tooth extraction on the oral microflora (De Waal et al. 2015, METC
2012.097) will be contacted for participation in the present study. During the
5 years follow-up examination microbiological samples from various locations in
the oral cavity will be obtained in the same manner as it has been done during
the previous examinations. Additionally, patients will be asked for permission
to use the previously collected samples for new analyses.
Study burden and risks
There are no risks involved with participation in this studie. To minimize the
burden, the research appointment will be
combined with regular visits to the patients own dentist if possible.
Antonius Deusinglaan 1
Groningen 9713 AV
NL
Antonius Deusinglaan 1
Groningen 9713 AV
NL
Listed location countries
Age
Inclusion criteria
- the patient has participated in the previous study (METC 2012.097)
- the patient is capable of understanding and giving informed consent
Exclusion criteria
- Chemotherapy in the previous 6 months;
- Systemic or local use of antibiotics in the oral cavity during the last 3 months;
- Use of anti-septic / antimicrobial therapies (including mouthrinses) in the oral cavity during the last 4 weeks;
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL58377.042.16 |