Verification of functional tests with the LoRRca, a next generation ektacytometer, on red blood cells of healthy individuals with or without comorbidities/risk factors.
ID
Source
Brief title
Condition
- Other condition
- Cardiac disorders, signs and symptoms NEC
- Glucose metabolism disorders (incl diabetes mellitus)
Synonym
Health condition
normale populatie, en populatie met comorbiditeit of risicofactoren
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Determination of reference values of the different functional tests the LoRRca
is able to perform, and the identification of comorbidities that can influence
test results of the LoRRca.
Secondary outcome
Not applicable
Background summary
Ektacytometry is a method which can be used to investigate red blood cell
membrane disorders. Laser Optical Rotational Cell Analyser (LoRRca) is a next
generation ektacytometer which can be used to measure different aspects of red
blood cell (RBC) deformability. Examples are deformability and aggregation
which can say something about the stability and rheology of RBCs.
Red blood cells have to be highly deformable as they need to be able to pass
through microcappillairies at high shear rate*s. Surface/volume ratio, pH,
osmolarity, viscosity and membrane structure can influence red blood cell
deformability. Many disorders alter one or more of these parameters and
therefor influence deformability and rheology.
Most research has been done with aggregation and deformability assays. Limited
research has been done with other of the different modalities the LoRRca is
able to perform. In particular, a new modality has very recently been
developed, enabling the study of RBC deformability under changing levels of
oxygenation.
In order to use this instrument a study must be done in order to determine
reference values and confounders of these reference values.
Study objective
Verification of functional tests with the LoRRca, a next generation
ektacytometer, on red blood cells of healthy individuals with or without
comorbidities/risk factors.
Study design
Cross-sectional observational study
Study burden and risks
The study will investigate blood of (healthy) volunteers. If possible the
venipuncture will be combined with a routine visit. Otherwise one venipuncture
is needed. The subjects will not benefit from this study.
De Corantijn 13
Zwaag 1689 AN
NL
De Corantijn 13
Zwaag 1689 AN
NL
Listed location countries
Age
Inclusion criteria
* 55-65 years (10), 20-30 years (40) or neonates (10)
* 55-65 years old, smoker (*-1 pack a day) and no other comorbidities (10)
* 55-65 years old and one of the following comorbidities: hypertension (10) or diabetes mellitus (10)
* 20-30 years old and obese (BMI >30) (10)
* Be able to give informed consent
Exclusion criteria
* Suffering from a serious condition not mentioned above
* Fever at time of venepuncture
* Not able to give informed consent
* Heavy alcohol drinking (more than 4 units a day)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL55333.041.15 |