To investigate associations between IBD severity, ASCA titer, microbiota composition and C. albicans strains present in feces.
ID
Source
Brief title
Condition
- Gastrointestinal inflammatory conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
1) A correlation in CD patiënts between C.albicans strains and ASCA titer in
blood.
2) A correlation in CD patiënts between fecal microbial composition and ASCA
titer in blood.
Secondary outcome
The secondary study parameters are based on a correlation between disease
severity (measured by determination of fecal calprotectin) and fecal microbial
composition/ fecal C.albicans strains in CD, UC and healthy volunteers.
Background summary
Various studies have suggested a role for fungi in IBD(1-3). 50% to 70% of
Crohn*s Disease (CD) patients produce antibodies against the mannan component
of fungi, called anti- saccharomyces cerevisiae antibodies (ASCA*s), and
against other fungal antigens. Only 5-15% of the ulcerative colitis (UC)
patients and 0-5% of healthy controls test positive for these antibodies. ASCA
titer seems also related to disease severity in CD, suggesting that fungi are
relevant for development of CD. A quarter of the intestinal fungal species are
of the Candida genus. It has been shown that in IBD patients fungal diversity
is reduced compared to healthy controls while the proportion of C. albicans is
increased. Together, this suggests that C. albicans plays a role in intestinal
inflammation in CD patients. C. albicans is a commensal fungus that can become
pathogenic. Information on genetic diversity and dynamics of the C. albicans
population and on the characteristics of C. albicans strains in healthy people
and in patients is important in order to clarify the role of C. albicans in
IBD. In collaboration with Prof. T. Broekhout (UVA and CBS-KNAW) we will carry
out multilocus sequence typing (MLST) of C. albicans isolated from stool of IBD
patients and healthy volunteers. MLST is a characterization technique in which
7 housekeeping genes are sequenced and based on SNPs located in these genes
15.000 C. albicans strains can be characterized(9). Besides the MLST of C.
albicans we will also perform 16S and 18S sequencing to map correlations with
the overall microbiota and mycobiota, also blood will be analyzed for the
presence of ASCA*s. Together this enables us to find correlations between
C.albicans strains, disease and presences of ASCAs in IBD patients.
Study objective
To investigate associations between IBD severity, ASCA titer, microbiota
composition and C. albicans strains present in feces.
Study design
observational study
Study burden and risks
This study is considered to be an observational study with a low patient risks,
all subjects will undergo already established procedures which include one
blood withdraw and handing of one faecal sample
Meibergdreef 69
Amsterdam 1105 BK
NL
Meibergdreef 69
Amsterdam 1105 BK
NL
Listed location countries
Age
Inclusion criteria
For all groups:
- Age from 18 years, either male or female
- Ability to give informed consent
- No use of antibiotics or antifungals 3 months prior to sample collection. ;Group specific inclusion criteria:
- Group 1 and 2 the patients have been diagnosed with CD.
- Group 3 patients have been diagnosed with UC patients.
- Group 4 People have not been diagnosed with IBD.
Exclusion criteria
Inability to give informed consent
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL63207.018.17 |