The primary objective of this study is to measure mitochondrial capacity and maximal oxygen consumption (VO2max) in endurance-trained and untrained young males in order to verify if NIRS can be used to analyse differences in mitochondrial function…
ID
Source
Brief title
Condition
- Other condition
- Muscle disorders
Synonym
Health condition
Veroudering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The main study parameter is the rate of recovery of muscle oxygen consumption
measured using NIRS combined with transient arterial occlusions. The rate of
recovery is expressed as a time constant. Subjects will be screened for
VO2max, measured using an incremental exercise protocol on a bicycle ergometer.
Eight endurance-trained and eight untrained subjects will be included based on
their VO2max. These subjects are invited to a study test day, in which they are
asked to arrive in a fasted state for a blood sample . Afterwards mitochondrial
capacity will be measured in the gastrocnemius and wrist flexor muscles using
transient arterial occlusions and NIRS measurements.
Secondary outcome
EPOC will be measured following a short sub-maximal exercise bout on a bicycle
ergometer by indirect calorimetry. Mitochondrial function will be measured in
PBMC*s extracted from blood using the Seahorse XFe analyzer.
Background summary
Analysing mitochondrial muscle health is challenging and current methods rely
on invasive methods, such as taking biopsies. Near-infrared spectroscopy (NIRS)
is a novel technique that allows us to measure changes in oxygenation states in
the muscle in vivo and non-invasively. Combining transient arterial occlusions,
by applying external pressure using a blood pressure cuff, with NIRS
measurements allows us to measure oxygen consumption in the muscle. This
technique will be used in future studies to measure changes in mitochondrial
function in response to dietary and exercise interventions in subjects in which
biopsies are often not preferred, such as in elderly subjects. Investigating
less invasive techniques will facilitate research in the future and could
reduce discomfort of the research for participants.
Study objective
The primary objective of this study is to measure mitochondrial capacity and
maximal oxygen consumption (VO2max) in endurance-trained and untrained young
males in order to verify if NIRS can be used to analyse differences in
mitochondrial function in a normal population. Mitochondrial capacity will be
measured in the gastrocnemius and wrist flexor muscles to compare which site
correlates best to measurements of whole body oxygen consumption. Furthermore,
the current protocol is designed to measure mitochondrial function in
peripheral blood mononuclear cells (PBMCs) and excess post exercise oxygen
consumption (EPOC) in order to verify if these techniques can be used in future
studies to assess mitochondrial capacity.
Study design
Cross sectional study
Study burden and risks
No direct health benefit for the subjects is expected. The experimental
procedures are safe, but can be painful or cause discomfort to a certain
degree. Blood sampling can cause local hematoma or bruising. The maximal
exercise test can cause muscle soreness. The mitochondrial capacity test makes
use of arterial occlusions by external pressure, which can be painful or
uncomfortable and possibly could bruise the site of the occlusion. The subject
is asked to fast overnight for the second day and remain fasted throughout the
test session. The benefits of participation are a relevant contribution to
scientific research and a financial compensation of ¤20 when completing the
screening test day and an additional ¤40 euros when completing the study test
day as well.
De Elst 1
Wageningen 6708WD
NL
De Elst 1
Wageningen 6708WD
NL
Listed location countries
Age
Inclusion criteria
18-28 year old male
BMI 18,5-25 kg/m2
VO2max * 47 mL/kg/min or VO2max * 57 mL/kg/min
Exclusion criteria
- Health concerns regarding respiratory and pulmonary diseases, such as COPD, (exercise induced) asthma and cardiovascular disease.
- (known symptoms of) Metabolic diseases, such as type I or II diabetes,
- Regular smoker (defined as smoking >5 cigarettes per week)
- Haemoglobin concentrations below 8.0 mmol/L
- Recent usage (within four months) of supplements with suggestive training effects, such as creatine phosphate, EPO or anabolic steroids.
- Usage of recreational drugs, such as marihuana, amphetamines and cocaine during the study (starting after screening day)
- Suffers from (sport) injury that hampers maximal exercise performance
- Blood donation during the course of study
- Current participation in other clinical trials
- Employed or undertaking a thesis or internship at the department of Human and Animal Physiology
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL60823.081.17 |
| OMON | NL-OMON22088 |