Primary; • To study the mitochondrial respiration in human BATSecondary;• Apply histological protein stainings and mitochondrial content analysis of human brown adipose tissue • Relate mitochondrial activity and content to the amount of UCP1, body…
ID
Source
Brief title
Condition
- Other condition
- Appetite and general nutritional disorders
Synonym
Health condition
obesitas
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The primary endpoint will be the quantification of mitochondrial respiration in
human BAT. Secondary endpoints will be histological protein stainings and
mitochondrial content analysis of human BAT. An UCP-1 immunofluorescence assay
will be used to detect UCP-1.
Secondary outcome
none
Background summary
Obesity is considered as a chronic disease in the modern western society. It is
associated with many comorbidities, enormous health costs and a decrease in
length and quality of life. Searching for a cause of the increase in obesity,
explaining this development in a metabolic perspective shows new insights.
Serendipity caused a renewed interest in the metabolic value of brown adipose
tissue (BAT), after it showed to be active on FDG-PET-CT-imaging. Recently BAT
showed to be present and active in adult human, with a decreased activity in
obesity.
After showing active BAT in man, its metabolic function needs further
investigation. Human BAT needs to be evaluated in its activity and physiologic
functionality in the human body as a whole. In this way the purpose of BAT can
be determined. Therefore we want to propose to study human BAT on a cellular
level using mitochondrial respiration techniques. In this way human BAT
activity in different subjects can be evaluated. Varying body compositions and
ages will be included to create a diverse data pool.
Study objective
Primary;
• To study the mitochondrial respiration in human BAT
Secondary;
• Apply histological protein stainings and mitochondrial content analysis of
human brown adipose tissue
• Relate mitochondrial activity and content to the amount of UCP1, body fat
percentage and body mass index
Compare activity and histology of BAT with white adipose tissue (WAT) and
skeletal muscle
Study design
Analysis of operative BAT, WAT and skeletal muscle biopsies of the
supraclavicular and neck region.
Patients indicated for surgery in the supraclavicular and neck region,
specifically patients diagnosed with hyperparathyroidea, struma, benign thyroid
gland tumours, implantation of a vagal neurostimulator for epilepsy and
cervical surgery for neurologic pathology will be included.
Study burden and risks
Routine surgery is performed with the addition of a BAT,WAT and skeletal muscle
biopsy. There is no increase of peroperative morbidity and mortality for the
patient.
Informed consent will be obtained. The only extra intervention consists of
taking one extra blood sample. This will be done together with the blood
sampling for routine purposes before surgery. The surgical operating field is
identical with or without biopsy. Previous studies have shown the highest
amount of active BAT in the neck and supraclavicular region. For this reason,
biopsies will be obtained from patients indicated for surgery in the neck. The
biopsy will be taken paying attention to the anatomical location of the
laryngeal nerve.
Postbus 616 Postbus 616
Maastricht 6200 MD
NL
Postbus 616 Postbus 616
Maastricht 6200 MD
NL
Listed location countries
Age
Inclusion criteria
Patients indicated for surgery in the supraclavicular and neck region are included. More specific, patients diagnosed with hyperparathyroidea, struma, benign thyroid gland tumours, implantation of a vagal neurostimulator for epilepsia and cervical neurologic disorders, aged 18-65 years will be included.
Exclusion criteria
Only patients indicated for elective surgery will be included. Patients undergoing acute surgery because of critical illness are excluded. In this patient group informed consent is difficult to obtain and severe inflammation could influence BAT-, WAT- and skeletal muscle responses. Patients with pre-operative mailgn tumours will be excluded.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL31367.068.10 |