The main objective is to determine the relationship between human attractiveness to mosquitoes and mosquito egg production. Secondary objectives are:* To determine the differences in human blood composition on mosquito egg production between and…
ID
Source
Brief title
Condition
- Protozoal infectious disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The primary study parameters are the relative attractiveness of odour samples
to mosquitoes and the number of mosquito eggs laid after taking blood.
Secondary outcome
Secundary study parameters are the concentrations of different blood
components, the immune response of the blood against certain pathogens,
differences in skin microbial profiles and the chemical compositions of odour
samples.
Background summary
Malaria is caused by the malaria parasite. A malaria mosquito can spread this
parasite when biting a human, if the mosquito has picked up the malaria
parasite from biting someone else before. A mosquito needs blood for
reproduction and some humans are more attractive to malaria mosquitoes than
others. We think that malaria mosquitoes can smell someone's blood composition
and that this is the basis for mosquito host preference.
Study objective
The main objective is to determine the relationship between human
attractiveness to mosquitoes and mosquito egg production.
Secondary objectives are:
* To determine the differences in human blood composition on mosquito egg
production between and within individuals
* To identify blood constituents that influence mosquito egg production
* To identify differences in skin microbial composition between individuals
that are highly attractive and poorly attractive to mosquitoes
* To identify differences in volatile production between individuals that are
highly attractive and poorly attractive to mosquitoes
Study design
This is a longitudinal observational study where each volunteer will be tested
five times in total; one intake and four times every five weeks during a
five-month period.
During the intake, each subject receives a questionnaire and will determine his
blood type using a finger prick test. At all four research visits, odour
samples, skin microbial samples, blood samples and a questionnaire will be
taken. Next, each subject will feed 20 not-infectious mosquitoes from the arm
during 10 minutes. These mosquitoes have not been in contact with the malaria
parasite and thus are not infective.
Study burden and risks
Risk and burden for the subject will be minimal. The volunteers will be exposed
to non-infectious malaria mosquitoes (the malaria parasite is not present in
our lab), so itching of max. 20 mosquito bites is the largest burden. The only
medical procedure will be taking blood samples where some pain may be involved.
Our healthy subjects will not benefit from the study themselves, but the
results will give more insight in mosquito host preference and mosquito
reproduction. This may, in time, lead to new malaria mosquito control tools.
Droevendaalsesteeg 1
Wageningen 6708PB
NL
Droevendaalsesteeg 1
Wageningen 6708PB
NL
Listed location countries
Age
Inclusion criteria
Man, 18-65 years old, healthy
Exclusion criteria
Woman, smoking, skin diseases such as eczema, BMI above 25, known to be
oversensitive to mosquito bites, regular use of medication, not available to
visit during specific time intervals, thesis student at the Laboratory of
Entomology, WUR
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL68436.081.18 |