The main aim of the current study will be to assess whether the ingestion of whey protein or casein protein stimulates overnight muscle protein synthesis following endurance-type exercise.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
spierherstel na inspanning
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Overnight myofibrillar protein synthesis rate
Secondary outcome
- Overnight mitochondrial protein synthesis rate
- Plasma amino acid concentrations
- Hunger VAS
- Sleep VAS
- Caloric intake at breakfast
Background summary
Previous research has shown that protein ingestion prior to sleep improves
overnight muscle protein synthesis rates following resistance exercise.
However, it is still unclear whether the type of protein ingestion influences
the overnight muscle protein synthetic response and whether protein ingestion
prior to sleep is also beneficial following endurance-type exercise.
Study objective
The main aim of the current study will be to assess whether the ingestion of
whey protein or casein protein stimulates overnight muscle protein synthesis
following endurance-type exercise.
Study design
The present study will be a double blind, placebo controlled, parralel,
intervention study.
Intervention
All subjects will perform a single session of endurance exercise in the
evening. Prior to sleep, subjects will ingest a whey-protein drink, a casein
protein drink, or a placebo drink.
Study burden and risks
The subjects will participate in 1 screening and 1 test night. They will be
required to fill out a medical questionnaire during the screening session. Also
a DEXA scan will be performed. There are no risks associated with the DEXA
scan. The radiation dose emitted during a DEXA scan is 0.001 mSv. This is a
very low exposure compared to the total background radiation in the
Netherlands, which is ~2.5 mSv/year. During the screening session a VO2max test
will be performed. On the testday two venous catheters will placed for
repeated blood draws and amino-acid infusion. The subjects will perform a
single session of endurance exercise in the evening of the testnight. Prior to
sleep, the subjects will drink an experimental test drink that contains whey
protein, casein protein, or a placebo drink. Following the ingestion of the
test drink, subjects will sleep for 7 hours. Prior to sleep, and upon waking, a
muscle biopsy will be taken, The subjects will be instructed to not perform an
intense physical labout in the 48 h prior to the testday en for 24 h prior to
the testday to not consume any alcohol, or to consume caffeine in 12 h prior to
the testnight. During the day of the testnight, subjects will consume
standardized meals that will be provided. There are no major risks of the
endurance exercise session other than the expected risks associated with
exercise performance (such as fatigue, fainintng and an abnormal blood
pressure). The protein drinks contain protein that are normally present in
nutrition (milk) and are safe for consumption.
Universiteitssingel 50
Maastricht 6229 MD
NL
Universiteitssingel 50
Maastricht 6229 MD
NL
Listed location countries
Age
Inclusion criteria
- Males
- Aged between 18-35 years
- Healthy
- 18.5 <= BMI <= 30 kg/m2
Exclusion criteria
- Smoking
- Sports/exercise > 4 sessions/week
- Lactose intolerant
- A history of neuromuscular problems
- Use of anticoagulation medication
- Recent (<1 y) participation in amino acid tracer [13C6 or 3,5-D2-tyrosine]
studies
- Individuals on any medications known to affect protein metabolism (i.e.
corticosteroids, non-steroidal anti-inflammatories, or prescription acne
medications)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL64719.068.18 |
| OMON | NL-OMON27053 |