To determine the difference in amino acid digestibility of milk, sorghum and black beans between older (65-80 years) and younger (20-35 years) adults using the dual tracer method.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
veroudering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Blood samples will be drawn every hour until the 5th hour and every 30 minutes
afterwards up to 8 hours. Plasma and meal amino acids enrichment will be
measured with gas chromatography - isotope ratio mass spectrometry (GC-IRMS)
for both isotopes. The ratio between 2H and 13C labelled amino acids in both
meal and blood plasma will be determined and amino acid digestibility of the
tested protein source will be calculated.
Secondary outcome
- To determine the difference in amino acid digestibility between milk, sorghum
and black beans within age groups.
- To explore the difference in blood amino acids kinetics between milk, sorghum
and black beans and between older and younger adults.
- To investigate the association between gut health as measured with blood
markers and amino acid digestibility of milk, sorghum and black beans.
Background summary
Older adults should meet their amino acid requirements to prevent development
of sarcopenia. Dietary protein quality is of importance, as determined by amino
acid composition and digestibility of the consumed protein. There is a need to
investigate the impact of ageing on amino acid digestibility and thus quality
in vivo. The dual tracer method is an indirect, minimal-invasive method to
determine amino acid digestibility in humans. It is expected that amino acid
digestibility decreases with age and that the effect between age groups is
greater for poorly digestible protein sources.
Study objective
To determine the difference in amino acid digestibility of milk, sorghum and
black beans between older (65-80 years) and younger (20-35 years) adults using
the dual tracer method.
Study design
A randomised cross-over study design with a dietary protein intervention will
be performed. The participants will have three test days, each separated by two
weeks. They will consume milk, sorghum and black beans on each test day
separately. The order of the protein sources is random.
Intervention
Twenty grams of three deuterium (2H) - labelled protein sources (milk, sorghum
and black beans) will be consumed on three different test days. The meals will
be divided into 10 portions, consumed every hour. The 2H-labelled protein
source is mixed with free 13C-labelled amino acids as reference.
Study burden and risks
Research in older adults is necessary to provide them with suitable nutritional
advice. The participants will not benefit directly from participating in this
study. Stable isotopes occur in nature, are not radioactive and are not harmful
for the subjects. Total blood sample of 450 mL divided over four weeks is not
expected to result in any side effects. The burden of this study concerns the
obliged consumption of three meals, blood donation and time investment of the
screening and three test days.
Stippeneng 4
Wageningen 6708WE
NL
Stippeneng 4
Wageningen 6708WE
NL
Listed location countries
Age
Inclusion criteria
- Age: 20-35 years or 65-80 years
- Body Mass Index (BMI): 20.0 - 30.0 kg/m2
- Veins suitable for blood sampling
- Healthy as assessed with a questionnaire
- Regular and normal Dutch eating habits as assessed with a questionnaire
- Having given written informed consent
- Willing to comply with study procedures
- Accept use of all encoded data, including publication, and the confidential
use and storage of all data for 15 years.
Exclusion criteria
- Chronic disease, for example:
o Diabetes mellitus, being treated for high blood glucose or increased fasting
blood glucose (> 6.7 mmol/l in finger prick blood)
o Active cardiovascular disease
o Hepatic disease (e.g. hepatitis)
o Renal disease
o Cancer
o Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding)
o Pancreatitis
- History of medical or surgical events that may affect GI function and the
study outcomes, for example:
o Bariatric surgery
o Gastrointestinal tract surgery
o Digestive tract disorder
o Chewing problems
- Medicine use that interferes with GI function and the study outcomes, for
example:
o Glucose lowering drugs
o Proton pump inhibitors
o Laxatives
- Habits that interfere with the study outcomes:
o Probiotics and/or protein supplement use
o Smoking
o Drug abuse
o Alcohol consumption for men >21 units/week and >4/day and for women >14
units/week and >3/day
o Following a weight-loss diet, medically prescribed diet or other diet with a
low calorie intake or an unbalanced nutrient intake like a vegan or very low
carbohydrate diet
o Moderate to high intense physical activity for more than 5 hours a week
o Difficulties with eating breakfast in the morning
- Other:
o Self-reported allergy or intolerance to the tested products (milk, sorghum,
black beans)
o Weight loss of more than 3 kg in the month prior to study screening
o Being pregnant or breastfeeding
o For men: Hb <8,5 mmol/l and for women: Hb <7,5 mmol/l
o Recent blood donation (<2 months prior to start of the study) and planning to
donate blood (<4 months after the termination of the study)
o Current participation in other research and <2 months previous participation
in other research
o Not having a general practitioner
o Not willing to accept information-transfer concerning participation in the
study, or information regarding his or her health, like laboratory results and
eventual adverse events to and from his general practitioner
o Working at the department of Human Nutrition and Health at Wageningen
University & Research
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL80529.091.22 |