The main objective of the current study is to assess the impact of dietary collagen supplementation on human tendon protein synthesis.
ID
Source
Brief title
Condition
- Tendon, ligament and cartilage disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Assess the impact of collagen supplementation on healthy tendon protein
synthesis in otherwise healthy individuals (18 - 40 y) scheduled for
ACL-surgery.
Secondary outcome
Assess the impact of collagen supplementation on injured ligament and healthy
muscle protein synthesis, based on ligament and muscle tissues that also become
available during the ACL-surgery.
Background summary
Tendons transfer the force exerted by the corresponding muscle to bones and,
therefore, are vital components of human locomotion. Tendon injury is very
common, both in athletes as the general population. Once injured, tendons
recover very poorly, resulting in a decrease in quality of life for the patient
and a substantial burden on the health care system. Therefore, interventions
that can aid in the prevention or treatment of tendon injury are warranted. The
growth of all tissues, including tendon, is regulated by the net difference
between protein synthesis and protein breakdown rates. Protein ingestion
stimulates protein synthesis. Collagen is the main protein in human connective
tissues, including tendon. The amino acids glycine and proline are the main
building blocks of endogenous collagen and vitamin C serves as a co-factor in
the synthesis and release of novel collagen proteins. Hence, dietary collagen
may be the preferred protein source to deliver high amounts of glycine and
proline and, together with vitamin C, maximize collagen synthesis in tendon.
However, no evidence is available on the effect of vitamin C enriched collagen
supplementation on human tendon protein synthesis.
Study objective
The main objective of the current study is to assess the impact of dietary
collagen supplementation on human tendon protein synthesis.
Study design
Double-blind, randomized trial with two parallel groups.
Intervention
All participants will be randomly assigned to the intervention or control
group. The intervention group will receive:
• Collagen supplementation: The vitamin C-containing collagen supplement (20 g
per dose) will be ingested as drink (100 mL of water mixed with 20 g of
collagen powder) twice daily, once at breakfast and once before sleep.
The control group will receive:
• Placebo: Vitamin C-enriched maltodextrin will be used as an isocaloric
placebo.
All participants will receive:
• Co-intervention: Short (5 min) hamstring or quadriceps exercise will be
performed by all participants, 30 - 60 minutes following test drink ingestion.
All participants will also ingest a small dose (20 mL) of deuterium oxide
daily, for the measurement of tissue protein synthesis.
Study burden and risks
The burden and risks involved in participating in this experiment are limited.
Participants need to ingest a test drink twice daily (100 mL per dose), need to
perform 5 minutes of low intensity exercise twice daily, need to ingest
deuterium oxide (20 mL) daily, and need to obtain a saliva sample daily, for
seven days. The ingestion of deuterium oxide has been applied in numerous
published studies and is entirely safe and non-toxic in the amounts provided in
the present study. Tissue collection will occur during ACL-surgery, which is
already planned as part of the participants* course of treatment. A graft of
the hamstring or quadriceps tendon will be used for ACL reconstruction, and a
sample will be obtained under anesthesia during the standard surgical
procedure. The tissue is extracted during the standard procedure, meaning that
patients will not take on any extra burden by participating in this study. Two
additional blood samples (2 x 10 mL) will be obtained before and after the
experimental period.
The data attained within this study may improve the practice of physicians,
physiotherapists, dieticians, and active individuals (including elite
athletes), by gaining more insight into the effect of collagen supplementation
on tendon protein synthesis, and thereby identifying a novel nutritional
strategy that can aid in the prevention and treatment of tendon injuries.
Heyendaalseweg 141
Nijmegen 6525 AJ
NL
Heyendaalseweg 141
Nijmegen 6525 AJ
NL
Listed location countries
Age
Inclusion criteria
Planned for ACL reconstruction with hamstring or quadriceps autograft
Age >=18 and <=40 years.
BMI >=18.5 and <=30 kg/m2
Exclusion criteria
• Blood donation during the study period or during the last 2 months
• Pregnancy
• Third generation oral contraceptives
• Consumption of >21 alcoholic beverages per week
• Use of any medications known to affect protein metabolism (i.e.
corticosteroids, non-steroidal anti-inflammatories, or prescribed acne
medications).
• Regular use of protein or other nutritional supplements (including vitamins).
• Reported slimming or medically prescribed diet
• Use of antibiotics in the past month
• Current participation in another biomedical research study.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL84264.096.23 |