Unravelling the *measles paradox* by in-depth characterization of the immune repertoire before and after natural measles or measles vaccination.
ID
Source
Brief title
Condition
- Viral infectious disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The main study parameter is the immune repertoire before and after measles.
Secondary outcome
The secondary parameters in this study are:
Comparison of humoral responses to various pathogens and the change over time
in samples taken before and up until 3 years after measles in comparison to
vaccination. This will also be compared 10 years after infection or vaccination
Profiling of peripheral cytokines and the change over time in samples taken
before and up until 3 years after measles in comparison to vaccination or
participants that remain uninfected. This will also be compared 10 years after
infection or vaccination.
Quantifying cellular responses by flow cytometry after antigenic stimulation
with a wide range of bacterial and viral pathogens and change over time in
samples taken before and up until 3 years after measles, in comparison to
vaccination, or participants that remain uninfected. This will also be compared
10 years after infection or vaccination.
Comparison of measles-specific immunity 10 years after infection or vaccination.
Correlation of above parameters to disease severity.
Background summary
Measles is caused by measles virus (MeV). The disease is associated with
lymphopenia and immune suppression, which is an important cause of
measles-associated morbidity and mortality. Measles immune suppression can last
several years, whereas measles lymphopenia is usually resolved within two
weeks. At the same time, measles induces lifelong immunity. This apparent
contradiction, known as the *measles paradox*, was partially solved when we
demonstrated that MeV infects and depletes pre-existing memory cells, thereby
causing *immune amnesia*. This model is supported by observations in animal
models and clinical studies. However, several questions remain to be addressed.
Study objective
Unravelling the *measles paradox* by in-depth characterization of the immune
repertoire before and after natural measles or measles vaccination.
Study design
Observational cohort study. We will perform phenotyping and functional analyses
of T- and B-cell (sub)populations in blood from individuals before and after
measles or measles vaccination to assess changes in the composition of the
immune repertoire.
Study burden and risks
This study is an observational cohort study in young adults that involves the
collection of clinical specimens before and after natural measles or measles
vaccination. A maximum of 50ml blood, a nose brush, and a nasosorption will be
obtained in 6 study visits. Negligible risks are associated with participation.
Participants will be informed of their measles immune status and advised to be
vaccinated if seronegative, with protection from measles in an upcoming
outbreak as potential benefit.
Dr Molewaterplein 40
Rotterdam 3015GD
NL
Dr Molewaterplein 40
Rotterdam 3015GD
NL
Listed location countries
Age
Inclusion criteria
Cohort A (18-25 years)
- No known history of measles or vaccination
- Decided to take MMR vaccination before the upcoming measles outbreak
Cohort B (18-25 years)
- No known history of measles or vaccination (seronegative confirmed)
- Contracted measles during the upcoming outbreak
Cohort C (18-25 years)
- No known history of measles or vaccination (seronegative confirmed)
Cohort D (18-25 years)
- Experienced measles during the 2013 outbreak
Cohort E (18-25 years)
- Received second dose of measles vaccines ±10 years ago
Exclusion criteria
1. Diagnosed chronic disease
2. Immune suppression (due to medication or underlying disease)
3. Additionally for subjects recruited to Cohort A:
-pregnant women or women planning to get pregnant in less than one
month after the start of the
study. This is a precaution; the MMR vaccine is not recommended for
pregnant women.
-People who have had a severe allergic reaction (e.g., anaphylaxis)
after a previous vaccination.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL86804.078.24 |