The objective is to initiate a hiPSC-resource from 10 genotyped participants of the Doetinchem Cohort Study, with a characterized life course history of up to 35 years during their adult life, with the intent to deploy mechanistic and causality…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
gezondheid en ziekten t.b.v. de volksgezondheid
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
- Ability to generate hiPSCs and differentiated derivative cells from healthy
somatic cells
- Ability to generate mutated cell lines using gene-editing
- Ability to study disease phenotype in mutated cell lines and compare it to
the control iso-genic cell lines
Secondary outcome
n.a.
Background summary
Mechanistic and causality studies are essential to validate epidemiologic
associations or suspected health effects of environmental exposures. Human
induced Pluripotent Stem Cells (hiPSCs) can be modelled in vitro and
differentiated into different cell types of the human body, allowing
mechanistic and causal studies in relevant target tissues. The genotyped
participants of the Doetinchem Cohort Study with their characterized life
courses (up to 35 years during their adult life) are an excellent starting
point to invite a genetic diverse set of participants to contribute to a
hiPSC-resource for scientific research regarding health and disease.
Study objective
The objective is to initiate a hiPSC-resource from 10 genotyped participants of
the Doetinchem Cohort Study, with a characterized life course history of up to
35 years during their adult life, with the intent to deploy mechanistic and
causality studies for scientific research regarding health and disease.
Study design
The Doetinchem Cohort Study is a cohort study with its origins in a random
sample of Doetinchem's population. Every 5 years, participants are invited for
research which consists of several questionnaires and a physical examination at
the GGD in Doetinchem. This proposal is an add-on to the current ongoing
measuring round 8 (METC-protocol nummer 23-169/X-A). As initiation and proof of
concept of the envisioned hiPSC-resource, we will seek a total of 10
participants of round 8 willing to donate a blood sample for iPSC-generation
for public health research.
Study burden and risks
Blood collection is a standard procedure, generally accepted, and also familiar
to the current participants. The sensation of blood collection can be
discomfortable for some participants, but the risk is considered
minimal/negligible. The benefits for the participants are limited: their
participation contributes to public health in general by gaining insights in
health and disease mechanisms.
Antonie van Leeuwenhoeklaan 9
Bilthoven 3721 MA
NL
Antonie van Leeuwenhoeklaan 9
Bilthoven 3721 MA
NL
Listed location countries
Age
Inclusion criteria
- Belonging to the subset of 5000 genotyped Doetinchem Cohort Study
participants.
- Already participated in the ongoing measurement round 8 of the Doetinchem
Cohort Study.
Exclusion criteria
- Cancer patients (past or present);
- Individuals knowingly having an HIV or Hepatitis C infection.
Design
Recruitment
Medical products/devices used
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL88899.041.25 |