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ID
Source
Brief title
Health condition
Diabetes, Insulin resitance and mitochondrial dysfunction
Sponsors and support
Intervention
Outcome measures
Primary outcome
- Peripheral glucose uptake (Rd)
- Resting, adp-stimulated and uncoupled mitochondrial respiration rates
Secondary outcome
-Skeletal muscle metabolites of plasma FFA, i.e. glycosphingolipids
- Plasma concentrations of adiponectin, insulin, cortisol, glucagon, catecholamines
Background summary
Recently, disturbed mitochondrial function was shown to be associated with free fatty acid (FFA)- and obesity induced insulin resistance. Different types of FFA can have differential effects on glucose metabolism. Therefore we evaluate the effects of elevated plasma monounsaturated vs. polyunsaturated fatty acids on glucose metabolism and mitochondrial function.
Study objective
We hypothesized that FFA loading could impair mitochondrial function and that the potency of FFA to disturb mitochondrial function and glucose uptake would depend on the degree of desaturation of the fatty acids infused.
Study design
Participants were studied in balanced assignment on three different occasions (each two weeks apart) during a 6 hours infusion of Intralipid ®, Clinoleic®, or saline 0.9% intravenously
Intervention
Infusion with with to different lipid emulsion respectivily Intralipid® and Clinoleic®, versus Saline. Each patient is his own control.
F5-162 Endocrinology and Metabolism
M. Brands
Meibergdreef 9
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5662663
m.brands@amc.uva.nl
F5-162 Endocrinology and Metabolism
M. Brands
Meibergdreef 9
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5662663
m.brands@amc.uva.nl
Inclusion criteria
1. Healthy male volunteer
2. Caucasian
3. Age between 18-35 years
4. Normal BMI ( 20-24.9 kg/m2)
5. VO2 max (90-110% predicted value according to Jones formula)
6. Stable weight during 3 months before participation and during participation
7. Normal physical activity with at least 2 times per week sport activities
8. Non-smoking
Exclusion criteria
1. Fasting blood glucose > 5,6 mmol/L or abnormal OGTT (2-hour value after oral ingestion of 75 g, glucose >7.8 mmol/l) Fasting insulin concentration >50 pmol/l
2. BMI> 25 and BMI< 20
3. DM type II in first degree relatives
4. All chronic diseases
5. Drug use
6. Dyslipidaemia
7. Abnormal liver or renal function
Design
Recruitment
Followed up by the following (possibly more current) registration
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Other (possibly less up-to-date) registrations in this register
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In other registers
| Register | ID |
|---|---|
| NTR-new | NL1439 |
| NTR-old | NTR1500 |
| Other | : MEC 05/295 |
| ISRCTN | ISRCTN wordt niet meer aangevraagd |