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ID
Source
Brief title
Health condition
Pancreatitis
Sponsors and support
Intervention
Outcome measures
Primary outcome
The ability to culture PDEC organoids from pancreatic duct biopsies.
Secondary outcome
- PDEC organoid characteristics (cellular and molecular composition)
- Ability to grow PDEC monolayers
- Ion transport (chloride and bicarbonate) in PDEC monolayers
- Ion transport (chloride and bicarbonate) in duodenal monolayers
- Carrier status for pancreatitis-related mutations
Background summary
Pancreatitis is a common cause of hospitalization, with no specific therapy available other than supportive care. Recent evidence indicates that the loss of electrolyte and fluid secretion by pancreatic ductal epithelial cells (PDECs) is a key factor in the etiology of pancreatitis. In particular, dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR) has been associated with pancreatitis. We hypothesize that organoids can be cultured from pancreatic ductal biopsies, obtained from chronic pancreatitis patients, to study electrolyte and fluid secretion. The potential role of ion transport abnormalities in the etiology of pancreatitis will be investigated in pancreatic and intestinal (duodenal) organoids, the latter an established model to study CFTR function.
Study objective
Organoids can be cultured from pancreatic ductal biopsies, obtained from chronic pancreatitis patients, to study electrolyte and fluid secretion.
Study design
Collection will be performed during pancreatoscopy.
Inclusion criteria
All patients undergoing pancreatoscopy within the framework of regular diagnostics or treatment.
Exclusion criteria
A potential subject that is younger than 18 years old will be excluded from participation in this study.
Design
Recruitment
IPD sharing statement
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL9772 |
| Other | METC Erasmus MC : MEC-2021-0737 |