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ID
Source
Brief title
Health condition
- Milk protein
- Protein digestion
- Muscle protein synthesis
Sponsors and support
Intervention
Outcome measures
Primary outcome
Muscle protein synthesis rates from 0-6 h following drink ingestion
Secondary outcome
- Muscle protein synthesis rates from 0-2 and 2-6h
- Plasma lysine concentrations
- Plasma amino acid concentrations
Background summary
Protein intake is an essential stimulus for muscle protein anabolism. The muscle protein synthetic response to protein ingestion is mainly determined by the post-prandial plasma amino acid response. Glycation of proteins during commonly applied milk processing procedures, attenuates the digestibility of a dairy product, and the subsequent appearance of amino acids in the circulation. The level of protein glycation in processed dairy products might therefore be an important modulator of the overall protein quality of a product, and its ability to stimulate protein metabolism. It has not yet been investigated if the glycation level of dietary protein modulates its capacity to stimulate muscle protein synthesis. Therefore, the current study will compare the muscle protein synthetic response after ingestion of a milk protein powder with different levels of protein glycation in healthy young men.
Study objective
Ingestion of low glycated milk protein results in a greater muscle protein synthetic response compared to high glycated milk protein.
Study design
- Muscle biopsies at t=0, 120, 360 min after drink ingestion.
- Plasma samples at t=0, 15, 30, 45, 60, 90, 120, 150, 180, 210, 240, 300, 360 min after drink ingestion.
Intervention
- Low-glycated milk protein + 2 grams leucine
- High-glycated milk protein + 2 grams leucine
- Water placebo
Inclusion criteria
- Males
- Aged between 18-35 years
- Healthy, recreationally active (participating in recreational sports activities ≥ 1 and ≤ 6 h per week, with a maximum of 2 h resistance-type exercise)
- 18.5 ≤ BMI ≤ 30 kg/m2
- No physical limitations (i.e. able to perform all activities associated with daily living in an independent manner).
Exclusion criteria
- Smoking
- Lactose intolerant or allergies to milk proteins
- Regular consumption of protein supplements (e.g. protein powders)
- Musculoskeletal disorders
- Metabolic disorders
- Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications).
- Chronic use of gastric acid suppressing medication or anti-coagulants
- Recent (<9 months) participation in amino acid tracer (L-[ring-13C6]-phenylalanine and L-[3,5-2H2]-tyrosine) studies
- Unstable weight over the last three months
- Diagnosed GI tract disorders or diseases
- Blood donation in the past 2 months
Design
Recruitment
IPD sharing statement
Followed up by the following (possibly more current) registration
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Other (possibly less up-to-date) registrations in this register
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In other registers
| Register | ID |
|---|---|
| NTR-new | NL8690 |
| Other | METC academisch ziekenhuis Maastricht/Universiteit Maastricht : METC20-011, NL72586.068.20 |