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ID
Source
Brief title
Health condition
/
Sponsors and support
Intervention
Outcome measures
Primary outcome
The primary outcome is microvascular blood flow expressed in arbitrary units measured in rest, following a bout of resistance-type exercise, immediately following the cold/thermoneutral water bath, and 60 and 180 min following the cold/thermoneutral water bath. Microvascular blood flow is measured with contrast-enhanced ultrasound.
Secondary outcome
Secondary outcomes include femoral artery blood flow expressed as L/min (measured with Doppler ultrasound at the same time points as microvascular blood flow) and L-[ring-13C6]-phenylalanine enrichment in mixed muscle protein expressed as mole percent excess measured with gas-chromatography-combustion isotope ratio mass spectrometry at 4 hours following the cold/thermoneutral water bath.
Background summary
Cold-water immersion (CWI) is a frequently applied strategy to accelerate post-exercise recovery in both recreational as well as professional athletes. However, recent insights suggest that CWI following a bout of resistance-type exercise can blunt recovery by lowering muscle protein synthesis rates. It has been speculated that CWI lowers muscle protein synthesis rates by restricting blood flow in and towards muscle tissue. The objective of this study is to assess the acute effect of CWI on muscle macro-, microvascular blood flow, and amino acid incorporation in muscle tissue during post-exercise recovery in healthy young males.
Study objective
We hypothesize that 20 min of one-legged CWI at 8 degrees Celsius (°C) will lower macro-, microvascular blood flow, and amino acid incorporation in muscle tissue following a bout of resistance-type exercise training when compared with the thermoneutral water immersed control (CON) leg (20 min at 30 °C).
Study design
Microvascular (primary outcome) and femoral artery (secondary outcome) blood flow will be assessed in rest, following a bout of resistance-type exercise, immediately following the cold/thermoneutral water bath, and 60 and 180 min following the cold/thermoneutral water bath. L-[ring-13C6]-phenylalanine enrichment in mixed muscle protein (secondary outcome) will be assessed at 4 hours following the cold/thermoneutral water bath.
Intervention
Cold-water immersion
Inclusion criteria
- Aged between 18-35 years
- Healthy, recreationally active
- Male
- BMI < 30 kg/m2
Exclusion criteria
- Smoking
- Allergies to milk proteins (whey or casein)
- Arthritic conditions
- A history of neuromuscular problems
- Recent (<1 y) participation in amino acid tracer studies
- Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications)
- Individuals with gastrointestinal diseases
Design
Recruitment
IPD sharing statement
Followed up by the following (possibly more current) registration
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In other registers
| Register | ID |
|---|---|
| NTR-new | NL9811 |
| Other | METC MUMC+ : METC 21-049 |