No registrations found.
ID
Source
Brief title
Health condition
1. Insulin resistance in non-obese fasting;
2. healthy human subjects.
Sponsors and support
Intervention
Outcome measures
Primary outcome
Insulin resistance, Free fatty acids and oxidative stress with and without acipimox.
Secondary outcome
Other measures of glucosehomeostasis: glucoregulatory hormones, (adipo)cytokines.
Background summary
During fasting hypoglycemia must be prevented to protect glucosedependent tissues. Insulin resistance is a mechanism to do so. Free fatty acids play a major role in this process. Also there is a role for oxidative stress that inhibits insulin mediated glucose uptake by several proposed mechanisms. Lipolysis can be inhibited bij acipimox wich will result in a decrease in FFA levels. Hypothetically FFA inhibition decreases oxidative stress with a concomitant decrease in insulin resistance.
Study objective
Elevated levels of Free Fatty Acids during fasting induce oxidative stress and cause insulin resistance to maintain euglycemia.
Study design
N/A
Intervention
Subjects will undergo a period of fasting and are assigned to receive either acipimox (inhibitor lipilysis) 250mg 4dd or placebo. Hereafter insulin sensitivity will be measured using stable isotope technique. Furthermore regulating hormones and lipids will be measured. Muscle specimens (v. lateralis) will be obtained for determination of intramyocellular lipids and transscription factors.
Department of Endocrinology & Metabolism, F5-162<br>
P.O. Box 22660
M.R. Soeters
Meibergdreef 9
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5669111
m.r.soeters@amc.uva.nl
Department of Endocrinology & Metabolism, F5-162<br>
P.O. Box 22660
M.R. Soeters
Meibergdreef 9
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5669111
m.r.soeters@amc.uva.nl
Inclusion criteria
1. 6 healthy men;
2. 18-38 years;
3. BMI 20-25;
4. stable weight during the last 3 months.
Exclusion criteria
1. Diabetes;
2. diabetes first degree relatives;
3. hypercholesterolemia;
4. high intensity sport activities;
5. positive oral glucose tolerance testing.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL476 |
| NTR-old | NTR517 |
| Other | : N/A |
| ISRCTN | ISRCTN85121743 |