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ID
Source
Brief title
Health condition
Young adults, Muscle protein synthesis
Sponsors and support
Intervention
Outcome measures
Primary outcome
The main study endpoint is the fractional synthetic rate (FSR) of muscle protein synthesis (myofibrillar proteins) from 0-5 hours in the post-prandial period.
Secondary outcome
-The fractional synthetic rate (FSR) of muscle protein synthesis (myofibrillar proteins) from -3-0, 0-2 and 2-5 hours in the post-absorptive and post-prandial period respectively.
-Whole-body protein kinetics (synthesis, breakdown, oxidation, net balance).
-Plasma enrichments (in MPE) of:
o L-[ring-2H5]-phenylalanine o L-[1-13C]-phenylalanine
o L-[1-13C]-leucine
o L-[ring-2H4]-tyrosine
o L-[3,5-2H2]-tyrosine
o L-[1-13C]-KIC
-Plasma total phenylalanine, leucine and tyrosine concentrations (expressed as μmol/L)
-Total plasma amino acids (AAmax [μmol/L])
-Plasma glucose (glucosemax [mmol/L])
-Plasma insulin (insulinmax [mU/L])
Background summary
Rationale: Conventional animal-based proteins such as meat (i.e. beef, pork, lamb), poultry, fish, eggs, and dairy are considered “high-quality” sources of dietary protein as they contain all of the essential amino acids (EAA) and are highly digestible. However, the production of sufficient amounts of animal-based protein from conventional sources to meet future global food demands represents a challenge. Edible insects have been proposed as an alternative source of dietary protein that can be produced on a viable and more sustainable commercial scale and, as such, may contribute to ensuring global food security. Many edible insects represent a rich source of protein, comparable to conventional meat and fish, and provide EAA in amounts comparable to certain high quality protein sources. However, there is currently limited data on the functional capacity of insect-based protein sources. Therefore, the aim of the present study is to assess the capacity of insect based proteins to stimulate postprandial skeletal muscle protein synthesis and support protein anabolism in vivo in humans to determine their nutritional quality when compared to a more conventional animal- based protein source.
Study objective
We hypothesise that mealworm protein ingestion after unilateral resistance exercise will lead to significant higher rates of muscle protein synthesis in the trained leg compared to the untrained control leg. We also hypothesise that these rates will be equivalent to muscle protein synthesis after ingestion of milk protein.
Study design
t=0, t=120, t=300 muscle biopsies
13 blood draws
Intervention
-Unilateral exercise bout with different protein drinks
T.A. Churchward-Venne
P.O. Box 616
Maastricht 6200 MD
The Netherlands
+31 43 388 2154
t.churchward-venne@maastrichtuniversity.nl
T.A. Churchward-Venne
P.O. Box 616
Maastricht 6200 MD
The Netherlands
+31 43 388 2154
t.churchward-venne@maastrichtuniversity.nl
Inclusion criteria
-Healthy males
-Age between 18 and 35 y inclusive
-BMI between 18.5 and 30 kg/m2
-Having given informed consent
Exclusion criteria
-Use of tobacco products
-Non-steroidal anti-inflammatory drugs (NSAID) in the 4 days prior to the experimental trial
-Allergies to milk proteins (whey or casein)
-Allergies to house dust mites or crustaceans
-Lactose intolerance
-Phenylketonuria (PKU)
-Blood donation within 2 months of study initiation
-Arthritic conditions
-A history of neuromuscular problems
-Previous participation in amino acid tracer studies
-Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications)
-Diabetes
-Training more than 5 days per week
Design
Recruitment
IPD sharing statement
Followed up by the following (possibly more current) registration
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL6897 |
| NTR-old | NTR7084 |
| CCMO | NL58529.068.16 |
| OMON | NL-OMON47452 |