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ID
Source
Brief title
Health condition
protein, metabolism, breath test, oxidation
Sponsors and support
Intervention
Outcome measures
Primary outcome
Percentage of given 30 g protein oxidized over 5,5 hours
Secondary outcome
Bloodsamples to measure:
Glucose
Insulin
C-reactive protein
Insulin-like growth factor 1
Background summary
Generally, there are two major fates for the amino acids derived from protein ingestion: 1) uptake into amino acid pool thereby being available for protein synthesis and 2) oxidation of excess amino acids. All amino acids that are oxidized, are not incorporated.
For the healthy adult population, a protein intake of 0.8 g/kg bw/day is recommended. However, stable renal transplant patients benefit from a higher protein intake of ¡Ý1.1 g protein/kg bw/day, suggesting higher protein requirements. Possible reasons for higher protein requirement could be the chronic use of corticosteroids in renal transplant patients, a low grade inflammatory state, or other. Corticosteroids are used to prevent graft failure, but also have side effects, such as muscle wasting/protein catabolism. Whether protein catabolism is altered in renal transplant recipients however, is unknown.
The question is whether in stable renal transplant patients higher levels of protein catabolism (oxidation) can be measured with the 13C-milk protein breath test, as compared to age-controlled healthy subjects.
Study objective
Ingested protein derived amino acids are used for the synthesis of new proteins. After the protein synthesis requirements are met, all surplus amino acids are oxidized as their is no storage for amino acids. Stable renal transplant patients benefit from a higher protein intake (>1,1 g/kg/day). One of the likely causes is the use of corticosteroids to prevent graft rejection. Corticosteroids lead to loss of lean body mass, which suggests disturbed protein metabolism. We suspect that stable renal transplant patients oxidize more of their ingested proteins compared to healthy controls. To test this hypothesis we will apply a 13C-milk protein breath test to measure whole body protein oxidation by measuring exhaled 13CO2:12CO2.
Study design
3 baseline breath samples before the breath test
t=0 consumption of test drink (30 g milkprotein dissolved in 500 ml water)
33 timepoints: a breath sample is given every 10 minutes, from t=10 to t=330 minutes
Intervention
No intervention is given prior to the breath test
Gerlof Reckman
Groningen 9713 GZ
The Netherlands
06 57 27 54 11
g.a.r.reckman@umcg.nl, g.a.r.reckman@pl.hanze.nl
Gerlof Reckman
Groningen 9713 GZ
The Netherlands
06 57 27 54 11
g.a.r.reckman@umcg.nl, g.a.r.reckman@pl.hanze.nl
Inclusion criteria
Age 18+
Male
Be able to consume a 500 ml test drink within 5 minutes
Be able to speak the Dutch language
Give written informed consent
Case: received renal transplant >6 months before the study
Stable medication schedule for >2 weeks before the study (e.g. steroid use)
Healthy control: donated kidney >6 months before the study
Exclusion criteria
Milk (protein) allergy or intolerance
Diabetes
Cancer, except skin cancer
Recreational drug use
Active infection (CRP >10)
Habitual average intake of more than 2 glasses of alcohol per day
Not able to stop alcohol consumption 2 days before the breath test
BMI <20 and >35
Design
Recruitment
Followed up by the following (possibly more current) registration
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL6707 |
| NTR-old | NTR6877 |
| CCMO | NL63147.042.17 |
| OMON | NL-OMON46350 |