No registrations found.
ID
Source
Brief title
Health condition
mitochondrial function, insulin resistance
mitochondrieel functioneren, insuline resistentie
Sponsors and support
Intervention
Outcome measures
Primary outcome
Insulin sensitivity (hyperinsulinemic-euglycemic clamp) and mitochondrial function.
Secondary outcome
1. Whole body oxidative capacity;
2. Body composition;
3. Skeletal muscle mitochondrial markers;
4. Muscle lipid accumulation;
5. Expression of proteins involved in fatty acid handling;
6. Insulin signalling in skeletal muscle.
Background summary
N/A
Study objective
Administration of a lipid emulsion consisting of long-chain triglycerides (LCT-trial) in non-trained individuals, will induce insulin resistance due to increased DAG accumulation and subsequent activation of the PKC pathway in skeletal muscle. Infusion of long-chain triglycerides (LCT-trial) in endurance-trained subjects will result in no -or less pronounced- insulin resistance, because of a better capacity to mobilize and/or oxidize fatty acids. In case of infusion of medium-chiain triglycerides (MCT-trial), the induction of insulin resistance will be absent for both, trained- and non-trained subjects.
Study design
The clamps will be separated by at least 1 week in each subject.
Intervention
All subjects will undergo three hyperinsulinemic euglycemic clamps, in a random order, with simultaneous infusion of either glycerol (control trial), LCT or MCT/LCT.
Department of Human Biology
Maastricht University Medical Center
Patrick Schrauwen
Department of Human Biology
Maastricht University Medical Center
Maastricht 6200 MD
The Netherlands
+31(0)43-388 15 02
p.schrauwen@hb.unimaas.nl
Department of Human Biology
Maastricht University Medical Center
Patrick Schrauwen
Department of Human Biology
Maastricht University Medical Center
Maastricht 6200 MD
The Netherlands
+31(0)43-388 15 02
p.schrauwen@hb.unimaas.nl
Inclusion criteria
1. Male sex;
2. Age 18-35 years;
3. BMI < 25 kg/m2;
4. Stable dietary habits.
Exclusion criteria
1. Family history of diabetes;
2. Medication use.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL1888 |
| NTR-old | NTR2002 |
| Other | METC Maastricht University Medical Center : 06-3-049 |
| ISRCTN | ISRCTN wordt niet meer aangevraagd. |