No registrations found.
ID
Source
Brief title
Health condition
Ischemia/reperfusion injury
ischemie/reperfusie schade
Sponsors and support
Department of Anesthesiology, Academic Medical Center Amsterdam
Intervention
Outcome measures
Primary outcome
- Cell damage such as LDH, apoptosis markers
- Expression of Cav-1 and Cav-3 in blood
Secondary outcome
- If Cav-levels are significantly different after helium inhalation, cells will be incubated with cav-antibodies
Background summary
Helium induces organ protection, but currently its mechanisms of action are unclear. This trial investigates the underlying mechanisms of helium-induced protection. We hypothesize that helium inhalation results in the release of "protective" mediators in the blood, which are transported to tissue at risk for ischemia/reperfusion damage. This is investigated in the current study in which healthy volunteers will breathe helium, after which blood will sampled. Different cells exposed to hypoxia/reoxygenation in vitro, will be incubated with this blood, and we will investigate whether a reduction in cell damage can be found.
Study objective
We hypothesize that inhalation of helium gas induces release of protective factors in the blood, which can be used to protect different cell types against ischemia/reperfusion injury.
Study design
timepoints blood sampling: T0 (baseline), T1 (directly after inhalation), T2 (6 h after inhalation), T3 (24 h after inhalation)
Intervention
- Inhalation of helium or air
- Blood sampling by venous puncture
Inclusion criteria
non-smoking, healthy male volunteers, aged 20-55 years
Exclusion criteria
-Active smoking or smoking in the previous six months
-Alcohol abuse or use of recreational drugs
-Any allergic reaction on medication in the past
-Presence of a chronic disease that is under current medical observation and needs pharmacological treatment, e.g. asthma, high blood pressure or diabetes
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL4367 |
| NTR-old | NTR4507 |
| Other | METC nummer : 2014_045 |
Summary results
preconditioning in the human endothelium in vivo. Anesthesiology 2013 Jan;118(1):95-104
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Oei GTML, Smit KF, van de Vondenvoort D, Wieland CW, Hollmann MW, Preckel B, Weber NC; The effect of helium inhalation on the innate and early adaptive immune system ex vivo. J Transl Med 2012;10:201
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Oei GTML, Huhn R, Heinen A, Hollmann MW, Schlack WS, Preckel B, Weber NC: Helium-induced cardioprotection of healthy and hypertensive rat myocardium in vivo. Eur J Pharmacol 2012; 684:125-131
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Berger MM, Huhn R, Oei GTML, Heinen A, Winzer A, Preckel B, Weber NC, Schlack W, Hollmann MW: Hypoxia induces late preconditioning in the rat heart in vivo. Anesthesiology 2010; 113:1351-60
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Oei GTML, Weber NC, Hollmann MW, Preckel B: Cellular effects of helium in different organs. Anesthesiology 2010; 112:1503-10.
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Huhn R HA, Weber NC, Kerindongo R, Oei GTML, Hollmann MW, Schlack W, Preckel B: Helium-induced early preconditioning and postconditioning is abolished in obese Zucker rats in vivo. J Pharmacol Exp Ther 2009; 329:600-7