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ID
Source
Brief title
Health condition
- Protein requirements
- Protein metabolism
Sponsors and support
Intervention
Outcome measures
Primary outcome
The estimated average protein requirement (EAR) and the recommended daily allowance (RDA) of the studied population.
Secondary outcome
Carbon dioxide production, 13CO2 production, plasma and urine L-[1-13C]-phenylalanine enrichments, and plasma and urine phenylalanine concentration in a young, healthy, male population when applying the IAAO technique.
Background summary
Dietary protein is essential for stimulating tissue renewal and growth. Current dietary protein guidelines are based on nitrogen balance studies, but the complexity of this method limits its widespread application to assess dietary requirements. The minimally invasive Indicator Amino Acid Oxidation (IAAO) technique was recently introduced as a valid way to assess dietary protein requirements. The first human study that has applied the IAAO technique to assess protein requirements was performed in 8 healthy adult men in 2008. Protein requirements defined by the IAAO technique were comparable to those defined by the nitrogen balance method. To date, this is still the only data available on protein requirements in healthy adult men as assessed by the IAAO technique. Therefore, purpose of this study is to perform a replication study to assess protein requirements in healthy adult men as assessed by the IAAO technique in a larger sample.
Study objective
We hypothesize that this replication study will obtain a similar estimated average requirement (EAR) for dietary protein around 0.93 g۰kg-1۰d-1, as previously determined in Canadian healthy young adult men.
Study design
Breath, urine & blood sample before tracer ingestion and at t = 120, 150, 180, 210 & 240 min after start of tracer ingestion.
Intervention
7 different protein intake levels:
- 0.1 g/kg/d
- 0.3 g/kg/d
- 0.6 g/kg/d
- 0.9 g/kg/d
- 1.2 g/kg/d
- 1.5 g/kg/d
- 1.8 g/kg/d
Inclusion criteria
- Male
- Aged between 18-35 years
- Healthy, recreationally active (exercise at least 1 per two weeks and maximum 4 days a week)
- 18.5 ≤ BMI ≤ 30 kg/m2
- No physical limitations (i.e. able to perform all activities associated with daily living in an independent manner).
Exclusion criteria
- Smoking
- Musculoskeletal disorders
- Metabolic disorders
- Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications).
- Chronic use of gastric acid suppressing medication or anti-coagulants
- Unstable weight over the last three months
- Diagnosed GI tract disorders or diseases
- Blood donation in the past 2 months
Design
Recruitment
IPD sharing statement
Followed up by the following (possibly more current) registration
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL9693 |
| CCMO | NL77716.068.21 |
| OMON | NL-OMON50939 |