In this pilot study we intend to measure Tregs in umbilical cord blood, in normal as well as in pregnancies complicated by preeclampsia, and will define their use as a predictor of the risk for developing allergic diseases in the child.
ID
Source
Brief title
Condition
- Allergic conditions
- Neonatal and perinatal conditions
- Respiratory disorders NEC
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The number of Tregs in the umbilical cord blood and in the blood of the mother.
Atopy of the mother.
Secondary outcome
Does not apply.
Background summary
Over the last few decades there has been an increase in prevalence of atopic
children. An interesting fact in this matter is that first born children are
more prone to become atopic than their later born siblings. The cause of this
effect remains unclear. Preeclampsia is a serious complication of pregnancy and
although the exact cause has not been identified, it is clear that there is an
immunological background. An observation made in preeclampsia is the fact that
women pregnant for the first time have a three times higher risk for developing
preeclampsia than women that have been pregnant before. We think regulatory T
cells (Tregs) are a candidate for regulating the above mentioned processes.
Study objective
In this pilot study we intend to measure Tregs in umbilical cord blood, in
normal as well as in pregnancies complicated by preeclampsia, and will define
their use as a predictor of the risk for developing allergic diseases in the
child.
Study design
The researcher will administer an interviewing questionnaire to the mother and
father, regarding questions on atopy, obstetric history and smoking. One tube
of blood will be drawn from the mother during a venapuncture for clinical
purposes. One tube of umbilical cord blood will be drawn after birth of the
placenta. The total time taken from the subjects is approximately one hour.
Participation in the study will not produce any risks for the subject.
In the blood of the mother specific IgE against inhalation allergens and
foodallergens will be identified using UniCap. In both the blood of the mother
and the child (cord blood) the number of Tregs will be identified using
FACS-analysis. After the delivery information about obstetric history, current
pregnancy and delivery will be collected from the clinical record. Analysis of
the data will be done using SPSS 12.
Study burden and risks
The time needed to fill out the questionnaire is approximately 30 minutes. No
further contact with the subject is required. No risks.
Hanzeplein 1
9713 GZ Groningen
Nederland
Hanzeplein 1
9713 GZ Groningen
Nederland
Listed location countries
Age
Inclusion criteria
Pregnancy of 26 weeks and more.
Exclusion criteria
> 1 fetus in current pregnancy
mmunological disorders: HIV+, AIDS, immunodeficiencies.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL11434.042.06 |