The aims of this proposal are to show that, additional to a normal daily food intake, the intake of carbohydrate and protein during and post-exercise increases muscle protein synthesis. Second, that the intake of carbohydrate and protein during…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
geen aandoening
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The additional increase in muscle protein synthesis in the carbohydrate plus
protein trial, compared to the carbohydrate and placebo trial, will be
determined by measuring the enrichment of amino-acid-isoptopes in muscle
biopsies and plasma samples.
Secondary outcome
The expected decrease in exercise-induced muscle damage, by the intake of
carbohydrate plus protein during exercise compared to the intake of
carbohydrate or water only, will be determined by measuring several markers of
muscle damage in blood plasma and muscle samples.
Background summary
In the absence of food intake, the post-exercise protein balance
(synthesis-breakdown) in the muscle is negative. The intake of protein, or
protein plus carbohydrate increases protein synthesis and reduces breakdown and
thereby creating a positive muscle protein balance. These results are obtained
in research with subjects in the fasted state, which means that they didn't
consume any food before or during exercise. This isn't representive for a
situation in which people practice sports in the evening after a day of normal
food intake. One could state that a normal daily caloric intake provides enough
substrate for muscles to recover from exercise. Our hypothesis is that, even in
the fed state, the intake of carbohydrate and protein during and after exercise
is important in post exercise muscle recovery and to diminish the rise in
muscle damage during exercise.
Study objective
The aims of this proposal are to show that, additional to a normal daily food
intake, the intake of carbohydrate and protein during and post-exercise
increases muscle protein synthesis. Second, that the intake of carbohydrate and
protein during exercise will diminish the exercise-induced muscle damage.
Study design
All subjects will perform two trials, separated by two weeks, in which he will
receive a differtent drink on each trial day. The subject will serve as his own
control. The drinks will be served in a randomised and double blind order.
Intervention
A trial begins with a 1 hour resting period, to get a steady state for the
amino acid tracer, followed by the first muscle biopsy. Then the training
protocol starts, which consists of a 40 min interval-cycling program and 80 min
resistance exercise. A second biopsy is taken and subjects are allowed to rest
supine for two hours, before they go to sleep. During exercise (every 15 min)
and at two timepoints post exercise (30 and 90 min) they receive a certain
amount of the test drink. Every half hour, and during exercise every 15 min, a
blood sample is taken from a heated hand vein. After a sleeping period of 7
hours the last muscle biopsy is taken. Stable amino-acid isotopes are given by
venous infusion during the entire test period.
Study burden and risks
The burden for the subjects consists of two half days and two evenings +nights.
The risks associated with participation are minimal. Muscle biopsies and
placement of the catheters can result in a local heamatoma. All procedures will
be performed with steril equipment and the muscle biopsies are taken by an
experienced physician.
Postbus 616
6200 MD Maastricht
Nederland
Postbus 616
6200 MD Maastricht
Nederland
Listed location countries
Age
Inclusion criteria
age 18-30
body mass index < 25 kg/m2
not engaged in any regular exercise programm
no use of medication
Exclusion criteria
female
other ages than indicated in the inclusion criteria
body mass index > 25 kg/m2
participation in any regular exercise program or resistance exercise
use of medication
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL11273.068.06 |