This study aims to identify qualitative and quantitative differences in innate, cellular and humoral immune response between persistent, intermittent and noncarriers of S. aureus.
ID
Source
Brief title
Condition
- Bacterial infectious disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
1. The amount of persistent, intermittent and noncarriers
2. The qualitative or quantitative difference in the presence of
antistaphylococcal IgG, IgM and IgA between healthy persistent, intermittent
and noncarriers of S. aureus in blood or nasal secretion
3. The qualitative or quantitative difference in the presence of CD4+ and CD8+
T cells between healthy persistent, intermittent and noncarriers of S. aureus
in blood
4. The difference in protein composition of nasal secretion between healthy
persistent, intermittent and noncarriers of S. aureus
5. The qualitative or quantitative difference in the presence of
antistaphylococcal antibodies and cellular immune response between healthy
individuals compared to patients with a bacteremia caused by S. aureus
Secondary outcome
Not applicable
Background summary
Staphylococcus aureus (S. aureus) is an important pathogen causing a variety of
infections ranging from mild to life threatening in the community as well as in
hospitals. The rise of MRSA has further increased the impact of S. aureus.
Carriers of S. aureus, about 20% of the healthy population, have an increased
risk of developing S. aureus infection. Carriers even have a three fold higher
risk for acquiring S. aureus bacteremia, but a significant lower risk of death
due to bacteremia compared to noncarriers. An explanation for this observation
has not been found yet, although a role for the immune response has been
proposed. Due to long time exposure, carriers may have developed a certain
level of immunity and possess protective antibodies and leukocytes. Noncarriers
may possess other antibodies and leukocytes, which protects them from becoming
a carrier. Infected patients are expected to display a high level of immune
response. Still, little is known about if, and if so, which immune mechanisms
are involved in S. aureus carriage and S. aureus infection however.
Study objective
This study aims to identify qualitative and quantitative differences in innate,
cellular and humoral immune response between persistent, intermittent and
noncarriers of S. aureus.
Study design
Cross sectional study. 400 healthy individuals are included.
On t0 a short questionnaire is filled in, a nasal swab and two bloodsamples are
taken. On t1 (one week later) a nasal swab is taken and nasal secretion is
collected.
Study burden and risks
The burden associated with participation is two short visits to the Erasmus MC.
The first time (t0) a short questionnaire is filled in, a nasal swab and two
bloodsamples are taken. Duration: 20 minutes.
One week later (t1) a nasal swab is taken and nasal secretion is collected.
Duration: 15 minutes. Total duration of study: 35 minutes.
There are no risks associated with filling in the questionnaire, taking a nasal
swab and collection of nasal secretion. The risk associated with the collection
of blood is a hematoma and pain near the ejection site. An experienced person
(not a student) will collect the sample.
's Gravendijkwal 230
3015 CE Rotterdam
NL
's Gravendijkwal 230
3015 CE Rotterdam
NL
Listed location countries
Age
Inclusion criteria
Healthy individuals older than 18 years
The volunteer has given informed consent
Exclusion criteria
Individuals with age below 18 years
Volunteers with diabetes mellitus, renal insufficiency, COPD, heart diseases, immunocompromised status (HIV, AIDS) or use of immunosuppressants, skin diseases like eczema and psoriasis.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL16312.078.07 |