To determine the frequency and suppressive potential of skin resident regulatory T cells isolated from atopic dermatitis and psoriasis patients compared to healthy control subjects (the latter data will be obtained by co-workers at the Brigham and…
ID
Source
Brief title
Condition
- Allergic conditions
- Epidermal and dermal conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
(a) Differences between the suppressive potential of skin resident regulatory T
cells isolated from atopic dermatitis, psoriasis patients and healthy control
subjects
(b) Differences between the the frequency of regulatory T cells in the skin of
patients with atopic dermatitis, psoriasis and healthy control subjects.
Secondary outcome
Not applicable.
Background summary
Atopic dermatitis (AD) is a common, itchy, chronic inflammatory skin disease. T
cells have been suggested to play a critical role in the pathogenesis of AD, and
lesional AD skin typically shows a striking infiltration of CD4+ T cells in the
dermis and epidermis.
Regulatory T cells constitute a small proportion of CD4+ T cells, but have been
shown pivotal for the suppression of immune responses. Recent, preliminary data
have shown increased percentages of regulatory T cells in the skin of AD
patients.
We therefore hypothesize that the chronic inflammation found in the skin of
atopic dermatitis patients results from impairment of function of regulatory T
cells.
After AD, psoriasis is the second most common skin disease, affecting
approximately 2-3% of the population worldwide. Also in psoriasis, T cells are
supposed to play a pivitol in the pathogenesis of the disease and regulatory T
cells have been shown in psoriasis skin lesions.
Study objective
To determine the frequency and suppressive potential of skin resident
regulatory T cells isolated from atopic dermatitis and psoriasis patients
compared to healthy control subjects (the latter data will be obtained by
co-workers at the Brigham and Women's hospital).
Study design
Observational study
Study burden and risks
More detailed knowledge about the characteristics of skin resident regulatory T
cells in AD and psoriasis will improve insights in the local inflammatory
processes and may lead to the development of new therapeutic strategies.
Skin biopsies are regularly taken in daily clinical practice and there are only
minor risks associated with it. Although a rare complication, infection of the
site of biopsy can be treated by the use of topical antibiotic ointments. The
wound resulting from the biopsy may leave a hypopigmented (small) scar.
Heidelberglaan 100
3584 CX
Nederland
Heidelberglaan 100
3584 CX
Nederland
Listed location countries
Age
Inclusion criteria
- adult (18-70 years of age), male or female patients diagnosed with atopic dermatitis (diagnostic criteria as described by Williams), or (guttate or plaque) psoriasis
- biopsy location (about 2x2 cm) should not be treated with topical steroids for at least 1 week
Exclusion criteria
- use of systemic immunosuppressive drugs (i.e., cyclosporin, prednisolone, methotrexate, neotigason, fumaric acid) in the 6 weeks prior to the inclusion
- exposure of biopsy location to (extraordinary) UV sunlight (i.e. UV-therapy, sunny holiday) in the 6 weeks prior to inclusion
- (secondary) skin infection
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL15763.041.07 |