The present proposal is designed to examine the differences in molecular signalling response to resistance versus endurance type exercise, leading to skeletal muscle protein synthesis.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
inspanningsfysiologie/spiergroei
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The difference in phosphorylation and activation of different signalling
proteins during and after resistance and endurance exercise.
Secondary outcome
The difference in blood glucose, insulin and amino acid concentration during
and after resistance versus endurance exercise.
Background summary
It has been well established that both endurance and resistance type exercise
activities lead to muscle protein synthesis in the post-exercise recovery
phase. Activation of the mTOR pathway is generally associated with resistance
type exercise, whereas the adaptation to endurance type exercise is generally
associated with the activation of the AMPK-PGC1α signalling pathway. However,
some studies suggest that the mTOR pathway is also involved in the synthesis of
muscle proteins after endurance type exercise activities. Research is warranted
to confirm these suggestions.
Study objective
The present proposal is designed to examine the differences in molecular
signalling response to resistance versus endurance type exercise, leading to
skeletal muscle protein synthesis.
Study design
Subjects will be tested on 2 occasions, seperated by 2 weeks. In the morning,
they will receive a standardized breakfast, rest for 2 hours and then perform
an endurance or resistance exercise session of 1 hour. Muscle samples will be
taken before, immediately after, and 4 and 22 hours after exercise to measure
the signalling proteins that are involved in the signal transduction pathway of
muscle protein synthesis. Subjects will receive standardized meals throughout
the day.
Intervention
The test starts with a standardized breakfast in the morning. This is followed
by a 2 hour resting period, whereafter the first muscle biopsy is taken and the
exercise protocol will start. This consists of 1 hour of resistance exercise or
1 hour of cycling exercise. At the end of the protocol a second muscle biopsy
is taken. This is followed by another resting period of 4 hours, after which a
third biopsy is taken. Subjects will receive a lunch at 12.00 and prepacked
dinner and snacks to take home. After an overnight fast, they will return to
the university the next morning at 9.00 am, and a fourth biopsy is taken,
whereafter aubjects will receive a breakfast.
Study burden and risks
The risks involved in participating in this experiment are minimal. Insertion
of the cathethers in a vein is comparable to a normal blood draw and the only
risk is of a small local haematoma. This is the same for the muscle biopsy. The
incision made for obtaining the muscle biopsy (performed by an experienced
physician) will heal completely.
Postbus 616
6200 MD Maastricht
Nederland
Postbus 616
6200 MD Maastricht
Nederland
Listed location countries
Age
Inclusion criteria
healthy men
Age: 18-28
Body mass index (BMI) < 25
Untrained
Exclusion criteria
Use of medication
BMI > 25
Enrolled in an endurance and/or strenght training program
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL22538.068.08 |