To examine the effects of odors and / or visual stimuli of food in subjects after 56h of starvation on:-Endocrine parameters-Metabolic parameters-Anthropometric parameters-Autonomic nervous system parameters-Hypothalamic function
ID
Source
Brief title
Condition
- Other condition
- Hypothalamus and pituitary gland disorders
- Glucose metabolism disorders (incl diabetes mellitus)
Synonym
Health condition
ouderdomsziekten en verouderingsproces
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
-Fasting endocrine and metabolic serum values
-Insulin sensitivity measured by oral glucose tolerance test (OGTT) (area under
the curve (AUC) of insulin, C-peptide, free fatty acid (FFA) and glucose)
-Glucose and FFA oxidation as determined by indirect calorimetry
-Hypothalamic response to oral glucose load as measured by fMRI (functional
magnetic resonance imaging)
-Anthropometric measurements (weight, waist and hip ratio (WHR), blood
pressure, bioelectrical impedance analysis (BIA))
Secondary outcome
none
Background summary
Calorie restriction (CR) can extend the lifespan and delay the onset of
age-related diseases of ad libitum fed organisms ranging from yeast to mammals.
In CR humans, promising results have been obtained. CR humans have favorable
risk factors for age-related diseases such as diabetes and cardiovascular
disease. In addition, CR humans and long-lived CR rodents have a strong overlap
in the physiological changes induced by the energy deficit. This so-called CR
response is characterized by a unique endocrine and metabolic status that has
been proven to mediate, at least parts of, the beneficial effects of CR.
Several studies have indicated that the brain is involved in mediating CR
longevity in diverging species. Specifically, neuronal energy-sensing has been
shown to be crucial for the retardation of aging by CR. Energy-sensing in the
brain depends on internal cues (glucose, insulin, leptin), but is also affected
by hedonic cues such as food-related stimuli. As the human hypothalamus plays a
central role in both energy metabolism and modulation of the endocrine system,
it might very well be the brain structure to elicit the human CR response.
The CR response of short-term CR and long-term CR seems to the same. Therefore,
studying short-term CR interventions can be used as a model to learn more about
the mechanisms of CR longevity.
In this protocol we want to investigate the role of energy sensing in the brain
on the CR response in humans by means of a 56 hour fasting period with or
without food-stimuli. Furthermore, we want to link the results to hypothalamic
function by neuro-imaging the hypothalamus during an oral glucose tolerance
test.
Study objective
To examine the effects of odors and / or visual stimuli of food in subjects
after 56h of starvation on:
-Endocrine parameters
-Metabolic parameters
-Anthropometric parameters
-Autonomic nervous system parameters
-Hypothalamic function
Study design
Randomized cross-over controlled intervention study
Intervention
56h fasting with and without food stimuli
Study burden and risks
The burden of 56 hour fasting en admission will logically be a feeling of
hunger. A healthy person will have no health risk of this intervention. The
collection of blood and fMRI will not lead to any noteworthy burden or risk
except for some dizziness caused by the fMRI scan.
albinusdreef 2
2333 ZA Leiden
Nederland
albinusdreef 2
2333 ZA Leiden
Nederland
Listed location countries
Age
Inclusion criteria
-Caucasian male volunteers
-Age > 18 years and < 70 years
-Body mass index (BMI) > 20 kg/m2 and < 25 kg/m2
-Fasting serum glucose < 6.1 mmol/L
Exclusion criteria
-Any significant chronic or hereditary disease (including renal, hepatic or endocrine disease)
-Any significant abnormal laboratory results found during the medical screening procedure
-Any use of medication (except for NSAID)
-Anosmia or any other (acquired) loss of olfaction (ea. rhinitis)
-Recent weight changes or attempts to loose weight (> 3 kg weight gain or loss, within the last 3 months)
-Difficulties to insert an intravenous catheter
-Smoking
-MRI contraindications
-Recent blood donation (within the last 3 months)
-Recent participation in other research projects (within the last 3 months), participation in 2 or more projects in one year
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL21132.058.07 |