The aim of the present study is to assess the necessity of dietary restrictions before sampling for the biochemical diagnosis of pheochromocytoma. Therefore, the short-term influence of a catecholamine-rich diet on plasma free and total metanephrine…
ID
Source
Brief title
Condition
- Adrenal gland disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Free and total metanephrines and free catecholamines are measured in both
plasma and urine.
Secondary outcome
Genotypes of enzymes in the catecholamine metabolic pathway.
Background summary
The clinical chemical diagnosis of pheochromocytoma is based on the
demonstration of an elevated catecholamine production rate. For this,
quantitative analysis of plasma and urinary free catecholamines (i.e.
epinephrine (E), norepinephrine (NE) and dopamine (DA)) and of their
metabolites, mainly the respective 3-O-methylated metabolites (metanephrines,
MNs) metanephrine (MN), normetanephrine (NMN) and 3-methoxytyramine (3-MT), are
routinely performed in many laboratories
Several food products contain substantial quantities of biogenic amines. For
example, DA and serotonin, and to a lesser extent NE, have been found in
relatively high amounts in bananas, pineapples and walnuts. Consumption of such
catecholamine-rich food products may increase plasma and urinary catecholamines
and each of their metabolites, and thereby produce false-positive test results
with respect to the diagnosis of pheochromocytoma.
Measurement of plasma free 3-O-methylated catecholamines (metanephrines) is the
current method of choice for diagnosing pheochromocytoma, but it is unknown
whether this procedure is affected by consumption of catecholamine-containing
food products.
Study objective
The aim of the present study is to assess the necessity of dietary restrictions
before sampling for the biochemical diagnosis of pheochromocytoma. Therefore,
the short-term influence of a catecholamine-rich diet on plasma free and total
metanephrine concentrations is investigated. Urinary MNs and plasma and urinary
free catecholamines are simultaneously measured in order to determine which
test is least influenced by dietary compounds, specifically catecholamine-rich
food.
Study design
Twenty healthy adults consume catecholamine-rich nuts and fruits (mainly
containing dopamine) at six fixed times within a single day. Timed blood and
urine samples are collected. Two days earlier the same protocol is carried out
with catecholamine-low products instead of catecholamine-rich products.
Blood is sampled in supine position after 30 min rest, by an intravenous
sampling line that is received by the participant in the morning to avoid
influence of sampling stress. Urine is collected over fixed time intervals
until the morning after the experimental day.
Isotope-dilution mass spectrometry is used for the quantification of
metanephrines and catecholamines.
Study burden and risks
During two days (9 hours per day) participants receive an intravenous line from
which at six fixed times blood is samples. During 24 hours urine is collected
divided over six fixed time intervals. The participants have to follow the diet
scheme on those two days. There are no risks for participating in this study.
Hanzeplein 1
9700 RB Groningen
Nederland
Hanzeplein 1
9700 RB Groningen
Nederland
Listed location countries
Age
Inclusion criteria
Healthy adults who gave written informed consent
Exclusion criteria
People that use certain medication that interrupt in the catecholamine-metabolism (like MAO-inhibitors, opiats and methyl-dopa). Also excluded from the study are non-healthy persons and pregnant women.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
Register | ID |
---|---|
CCMO | NL20324.042.07 |