To investigate whether the expression of renal water and sodium transporters in urinary exosomes is different in compensated versus decompensated liver and heart disease.
ID
Source
Brief title
Condition
- Other condition
- Heart failures
- Hepatic and hepatobiliary disorders
Synonym
Health condition
hyponatriemie
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Expression profiles of water and sodium transporters in urinary exosomes.
Secondary outcome
Not applicable.
Background summary
Since 1995 it has been known that renal transporters are excreted into the
urine (Kanno et al, N Engl J Med 1995). However, only in 2004 it has become
clear how these transporters are excreted into the urine (Pisitkun et al, Proc
Natl Acad Sci USA 2004). The renal transporters are present in so-called
urinary exosomes.
Study objective
To investigate whether the expression of renal water and sodium transporters in
urinary exosomes is different in compensated versus decompensated liver and
heart disease.
Study design
Patients will be recruited from the outpatient clinics and the clinics of
cardiology and hepatology. Compensated and decompensated disease will be
defined on the basis of the serum sodium level (cut-off 130 mmol/l). Healthy
volunteers will be recruited through untargeted advertisement. Blood and urine
will be collected via standardized procedures. Subsequently, urinary exosomes
will be isolated and the expression of water and sodium transporters will be
analyzed through immunoblotting.
Study burden and risks
Phlebotomy, burden: mild, risks: hematoma, vasovagal syncope.
Collection of urine, burden: minor, risks: none.
Postbus 2040
3000 CA Rotterdam
NL
Postbus 2040
3000 CA Rotterdam
NL
Listed location countries
Age
Inclusion criteria
All patients with heart or liver disease whose disease is not associated with renal disease and who are not taking medication that can cause kidney damage. Healthy volunteers without heart or liver disease, who are not taking medication.
Exclusion criteria
Patients with heart or liver disease whose disease is associated with renal disease or who are taking medication that can cause kidney damage. Healthy volunteers who are taking medication.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL26739.078.09 |