To demonstrate and compare expression of CCL25 and CCR9+ lymphocytes in peripheral blood and colon of PSC-, PSC/IBD-, IBD-, gastroenteritis patients and controls.
ID
Source
Brief title
Condition
- Gastrointestinal inflammatory conditions
- Hepatic and hepatobiliary disorders
- Autoimmune disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Expression of CCL25 in human colon by immunohistochemistry.
Secondary outcome
Difference in presence of CCL25 and/or CCR9+ lymphocytes in double stained
colonic biopsies between patients and controls.
Quantitative analyses of CCL25 mRNA expression in colon and CCR9+ T-cells in
peripheral blood of patients and controls.
Background summary
Primary sclerosing cholangitis (PSC) is a rare chronic cholestatic disease of
unknown cause. Chronic inflammation leads to bile duct destruction resulting in
liver failure. PSC is regarded as an immune dysbalance disease. PSC has a
strong association with IBD, especially ulcerative colitis and some consider
PSC as an extraintestinal manifestation of IBD. The gut-homing lymphocyte
paradigm offers a plausible explanation linking the gut and liver in PSC.
Study objective
To demonstrate and compare expression of CCL25 and CCR9+ lymphocytes in
peripheral blood and colon of PSC-, PSC/IBD-, IBD-, gastroenteritis patients
and controls.
Study design
Exploratory case control study.
Study burden and risks
Colonic biopsies are obtained during surveillance colonoscopy at the Department
of Gastroenterology and Hepatology AMC. 8 specimens at 3 levels, 24 specimens
in total per patient. During standard surveillance colonoscopy procedure 32
biopsies are obtained for pathological analyses. The additive burden consists
of 24 additional biopsies. One extra blood sample is drawn prior to
colonoscopy. Colonic biopsies are accompanied by a small risk of perforation,
bleeding and infection.
Postbus 22600
1100 DD Amsterdam
NL
Postbus 22600
1100 DD Amsterdam
NL
Listed location countries
Age
Inclusion criteria
Age: >18
Established diagnosis of PSC
Established diagnosis of IBD
Established diagnosis of infectious enterocolitis
Exclusion criteria
Bleeding diathesis
Inability to give informed consent
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL26892.018.09 |