To investigate in situ a possible effect of 4 different tootpastes on the developement of dental erosion and on the composition of the pellicle.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
tandaandoeningen
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The loss of enamel due to erosion will be determined using Atomic Absorption
Spectroscopy (AAS, calcium concentration) and molybdate UV-spectrophometry.
With these techniques the loss of enamel can be calculated. Baseline
measurements of the eroding solution will be performed. After exposing the
samples to the eroding solution the calcium concentration of the solution is
measured again. The differences in calcium concentration are an indication of
the loss of enamel [Dijkman et al., 1983; Vieira et al., 2005].
Secondary outcome
The pellicle adhered to the porcelain attached to the removable appliance will
be analysed using SDS-page. The differences in protein composition between a
pellicle exposed to toothpaste and normal pellicle will be determined
Background summary
Erosive wear is the loss of enamel due to a chemical process without the
involvement of bacteria. The main cause is the excessive consumption of acidic
drinks. Recently there is an increasing interest for and prevalence of dental
erosion in young people [Truin et al., 2004]. Saliva and pellicle composition
could be of importance in explaining this difference. The pellicle, a thin
layer of salivary proteins adsorbed to the tooth surfaces, has an important
function in the protection of the dental hard tissues. Erosion can be modified
by saliva and pellicle characteristics, such as saliva flow, pH, viscosity,
protein and mineral composition, buffer capacity and pellicle composition.
Moreover oral hygiene products such as toothpaste can influence the development
of dental erosion.
Study objective
To investigate in situ a possible effect of 4 different tootpastes on the
developement of dental erosion and on the composition of the pellicle.
Study design
It is a single centre, single blind study. 16 healthy volunteers will
participate in this study. The volunteers will carry a removable appliance with
4 pieces of human enamel (2 pieces foor exposure to toohpaste; 2 pieces as a
control group) and 2 porcelain pieces (for pellicle analysis) in the upper jaw
during a half day (13.30 - 16.00). Also 1 porcelain disc will be carried in the
buccal sulcus. After 1,5 hours in the mouth the enamel and the unattched
porcelain sample will be exposed to one of the tootpastes. 30 minutes later the
enamel and the unattched porcelain disc wil be exposed outside the mouth to an
eroding solution. One of the two porcelain pieces attached to the appliance
will be exposed to the toothpaste. These samples will be frozen for further
analysis. The loss of enamel will be determined using Atomic Absorption
Spectroscopy (AAS). With this technique the loss of calcium from the enamel can
be calculated [Dijkman et al., 1983; Vieira et al., 2005]. Moreover, the loss
of phosphate from the enamel and the fluoride concentration in the solution
after exposure will be measured. This procedure will be repeated for every
toothpaste.
Intervention
Enamel placed in an intra oral appliance will be brushed with 3 experimental
toothpastes and one conventional toothpaste. The volunteers wear these
appliances for 4 afternoons (1 toothpaste every afternoon).
Study burden and risks
The burden for a volunteer is to wear for proximally 3 hours a removable
appliance with enamel and porcelain pieces in the upper jaw and one unattached
porcelain disc in the buccal sulcus of the lower jaw during 4 afternoons.
Moreover, the volunteers are not allowed to drink, eat, brush teeth or smoke 1
hour before and during the test afternoon. swallowing of the enamel and
porcelain pieces/disc is a possible risk. The attachment of the enamel in a
removable appliance with composite is a proven safe method (Metc 2004.149). By
placing the unattached porcelain disc in the buccal sulcus the risk of
swallowing is reduced to a minimum.
Antonius Deusinglaan 1
9711 XB Groningen
Nederland
Antonius Deusinglaan 1
9711 XB Groningen
Nederland
Listed location countries
Age
Inclusion criteria
Adults with an ASA score of 1 with a rehabilited dentition.
Exclusion criteria
An ASA score of 2 or higher, severe periodontal disease (Dutch periodontal screening indez, DPSI > 2) and an unrehabiliated dentition. Participants wearing a removable appliance in the upper jaw can not participate.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL25020.042.08 |