To characterise the number, phenotype and functionality of regulatory T cells and cytokines produced in the peripheral blood of children diagnosed with asthma.
ID
Source
Brief title
Condition
- Respiratory disorders congenital
- Allergic conditions
- Bronchial disorders (excl neoplasms)
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Number, phenotype and functionality of regulatory T cells in the peripheral
blood.
Secondary outcome
Cytokine analysis in the peripheral blood
Background summary
Of all newborns, 33% suffer from at least one period of wheezing before the age
of 3 years. Of these so-called early wheezers (EW), only 40% will continue to
wheeze between the age of 3 and 6 years (persistent wheezers=PW). At the age of
6 years PW, also designated to have asthma, can be differentiated from
so-called transient wheezers (TW) who did not continue to wheeze between the
age of 3 and 6 years. Attempts to differentiate PW from TW in early infancy
have so far failed. In the related DART (diagnosis of asthma with regulatory T
cells)-project proposal we hypothesize that Tregs play a role in the
development of asthma and that TW and PW differ in either number, phenotype or
functionality of Tregs. In order to establish the best immunological parameters
and experimental assays to study in the DART-project it is important to first
identify the main differences, and ways to best identify these differences, in
number, phenotype and functionality of regulatory T cells and in cytokine
production between children diagnosed with asthma and healthy children.
Study objective
To characterise the number, phenotype and functionality of regulatory T cells
and cytokines produced in the peripheral blood of children diagnosed with
asthma.
Study design
A pilot study including in vitro studies on peripheral blood of children
diagnosed with asthma and of a healthy control group.
Study burden and risks
The risks and burden for subjects in this study are considered negligible.
Blood will be taken at the same time as routine blood withdrawal, as much as
possible. The subjects in the control group will be asleep/anaestitised at the
time of blood withdrawal. A maximum of 2 blood samples of 20ml during the whole
study will be taken.
Lundlaan 6
3584 AE Utrecht
NL
Lundlaan 6
3584 AE Utrecht
NL
Listed location countries
Age
Inclusion criteria
Diagnosis asthma for more than one year
Use of inhaled corticosteroids for more than one year
Lung function reversibility of more than 10%
RAST test performed
OR
No asthma
Exclusion criteria
The use of systemic immune modulating medication at the time of blood withdrawal and/or 6 weeks before blood withdrawal. Active infection at the time of blood withdrawal and/or the use of antibiotics at the time of blood withdrawal.
OR
Allergies and autoimmune diseases.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL19836.041.07 |