The main objective is to compare the electrophysiological differences and similarities between cardiomyocytes obtained by iPS and mature human cardiomyocytes.
ID
Source
Brief title
Condition
- Cardiac disorders, signs and symptoms NEC
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The two cell types will be characterized on a molecular and cellular
electrophysiological level.
Secondary outcome
na
Background summary
Because of recent development of new techniques in stem cells, it is possible
to reprogram somatic cells into an embryonic like state, the so called induced
pluripotent stem cells (iPS). The technique to differentiate stem cells to
cardiomyocytes is already accessible. This is a huge step forward towards new
cell models for cardiac diseases. However, little is known about the
differences or similarities and variability between mature human cardiomyocytes
and iPS derived cardiomyocytes (iPS-CM) on electrophysiological level e.g. ion
channels and ion currents. In this study we want to compare these two cell
types on molecular and cellular electrophysiological level to broaden our
knowledge about these cells and their suitability.
Study objective
The main objective is to compare the electrophysiological differences and
similarities between cardiomyocytes obtained by iPS and mature human
cardiomyocytes.
Study design
The two cell types will be collected by skin biopsy and ventricular puncture
during open heart surgery. By use of the patch clamp technique iPS-CM and
mature cardiomyocytes of 10 patients will be evaluated and compared.
Study burden and risks
Inclusion in the study does not bring additional burden of risk for these
patients.
Meibergdreef 9
1105 AZ Amsterdam
NL
Meibergdreef 9
1105 AZ Amsterdam
NL
Listed location countries
Age
Inclusion criteria
Patienten admitted for aortic valve replacement
Over 18 years and under 80 years old.
Exclusion criteria
The inclusion criteria includes only the study population. Since there is a minimal burden on patients, there is no reason why someone in this group can not participate in this study based upon exclusion criteria.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL33980.018.10 |