Protocol for study comparing skin autofluorescence, using the AGE reader, with fasting plasma glucose and glycated hemoglobin for the detection of diabetes mellitus or impaired glucose tolerance, with the use of the short glucose tolerance test as standard.

Detection of diabetes and impaired glucose tolerance (IGT) now depends on
suboptimal tools. Recently it has been shown that noninvasive SAF is superior
to plasma glucose and HbA1c in detection of diabetes as comfirmed by a glucose
tolernace test (GTT). Similar results have been found using SAF and a simple
decision tree in a group of high risk of diabetes persons for whom GTTs and SAF
were available. Preliminary cut-off values for SAF and the decision tree have
been made. The aimm of the current proposal is to test the SAF and used
cutt-off values of SAF and decision tress in an intermediate risk of diabetes
group.

The aim of the current study proposal is to test the validity of a decision
tree focused on skin autofluorescence, and based on the results described
above, as a detection tool for diabetes and impaired glucose tolerance in 2 new
cohorts, one in an outpatient based intermediate risk group, the other in a
low-risk primary care group.

Comparative study of SAF, fasting and historical plasma glucose and HbA1c for
detecting GTT-detected diabetes and IGT in persons at intermdiate risk
recruited initially in the UMCG Internal Medicine ooutpatients. If good test
characteristics of SAF and decision tree are confirmed A separate second studiy
will be done in lower risk primary care persosn (taking this second step will
be reported to METc via an amendment)

GTT is usually done in the study subjects. The noninvasive and rapid, safe SAF
measurement is not burdensome to the participants.