To determine the effectiveness of sputum induction in retrieving good quality sputum, identifying bacterial and viral pathogens in lower respiratory tract infections and determine the practical applicability of sputum induction in general pediatric…
ID
Source
Brief title
Condition
- Respiratory tract infections
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The quality of induced sputum, defined as <25 squamous cells and >25 leukocytes
per visual field under light microscopy.
Secondary outcome
To identify bacterial and viral pathogens of lower respiratory tract infections.
To determine the practical applicability of sputum induction in general
pediatric practice.
To determine the prevalence of Coxiella burnetii infections (Q fever) in
children with lower respiratory tract infections.
To determine the prevalence of Influenza A type H1N1-infections in children
with lower respiratory tract infections.
Background summary
Lower respiratory tract infections are a major disease burden in children and
give an annual death rate of 2 million world wide. In most cases, the pathogens
are not identified, current dia
(Current diagnostic tests provide little differentiation between bacterial and
viral pathogens, and in most cases the pathogens are not identified. )
Adults can produce sputum that can be analysed for pathogens, but in children
this is often not possible because they cannot produce sputum. By inhaling
hypertonic saline solution, sputum production increases and a coughing reflex
is prompted. This study investigates the effectiveness of sputum induction in
retrieving good quality sputum in children with suspected lower respiratory
tract infections.
Study objective
To determine the effectiveness of sputum induction in retrieving good quality
sputum, identifying bacterial and viral pathogens in lower respiratory tract
infections and determine the practical applicability of sputum induction in
general pediatric practice.
Study design
A prospective cohort study.
Study burden and risks
Sputum induction is a non-painfull procedure but can be experienced as
discomforting. Complications are rare and consist of epistaxis, vomiting,
transient wheezing and mild, reversible hypoxia (oxygen saturation 88-92%).
During the routinely performed venipuncture, extra blood is collected. If no
blood was collected during routine investigations, a venipuncture will be
performed. Urine is also collected, if necessary by collection bag. After 4
weeks, another venipuncture is performed for convalescent serology testing. If
the above mentioned procedures can identify the pathogens, current
overtreatment with antibiotics can be decreased and hospital admissions can be
shortened or even prevented. If bacterial pathogens are determined during the
intervention, the current antibiotic treatment of the study subject can be
altered, which is of direct benefit to the study subject.
Tolbrugstraat 11
5211 RW
NL
Tolbrugstraat 11
5211 RW
NL
Listed location countries
Age
Inclusion criteria
Tachypnea combined with at least one of: elevated body temperature, cough, dyspnea or abdominal pain
Exclusion criteria
- Acute exacerbation of asthma in the last 4 weeks. This is defined as requiring systemic coricosteroids, hospital admission and/or use of ß-sympaticomimetics >400 µg per day
- Oxygen saturation < 92% without oxygen therapy or severely ill impression
- Anatomical abnormalities of the respiratory tract presenting with inspiratory stridor
- Use of β-blockers, diuretics or theophylline
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL28965.028.09 |