The primary aim of the study is to determine the digestion kinetics of the two potato proteins and to compare these kinetics with casein and whey protein, two frequently used food proteins. The digestion kintetics will be determined using blood…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
Geen aandoening, maar de kinetiek van absorptie in gezonde proefpersonen
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Blood profiles of total and individual amino acids in time as a measure of
protein digestion and absorption kinetics
Secondary outcome
Blood glucose and insuline levels in time as a measure of the insulinotropic
response of the potato proteins
Tertiary outcome: Satiety hormones (only to be performed if blood amino acids
profiles are comparable to whey protein) to assess the effect of protato
proteins on satiety.
Background summary
The kinetics of protein digestion determines the kinetics of appearance of
amino acids in blood. Differences in kinetics in protein digestion are related
to bioavailability of amino acids for synthesis of muscle protein, regeneration
of muscle glycogen (important after physical excercise) and satiety. In
addition, insight into the blood amino acid profiles is important for the right
use in functional foods. The digestion kinetics of two potato proteins, the
high molecular weight fraction (HMW) and the low molecular weight fraction
(LMW), is not known.
Study objective
The primary aim of the study is to determine the digestion kinetics of the two
potato proteins and to compare these kinetics with casein and whey protein, two
frequently used food proteins. The digestion kintetics will be determined using
blood amino acid profiles in time.
The secundairy aim is to determine the effect of the two potato proteins on
blood insulin and glucose levels to gain insight into the insulinotropic
effects of the two potato proteins.
Finally, the tertiary aim is to determine the effects of the two potato
proteins on blood satiety hormones, in order to test the potential for satiety
products. This will only be performed if the postprandial blood amino acid
profile is comparable to that of whey protein.
Study design
In a double-blinded cross-over study, the following 4 pateurized protein drinks
(20 g protein in 500 mL protein- and sugar free sports drink; matrix with a
non-caloric sweetner, flavors, vitamins and stabilisers) will be tested in
fasted subjects:
1) whey protein (reference drink with fast digestible protein; positive
control); 2) high molecular weight fraction potato protein (HMW); 3) low
molecular weight fraction (LMW); 4) casein (reference drink with slow
digestible protein). The test days will be separated by a wasout period of 1
week. The test drinks will be randomized in time.
Blood (5 mL) will be samples every 15 minutes, starting 15 minutes before
drinking the test drink. After the second blood sampling, the drink will be
provided,The last sample will be drawn 3 h after the ingestion of the test
drinks. To this end, a vein in the underarm will be canulated.
Each intervention day, 14 blood samples of 5 m each will be drawn. The total
blood sampling per day wil be 70 mL. the total blood sampling during the whole
period will be 280 mL over 4 weeks.
The design is comparible, but not identical to a design by Farnfield et al.
(2009)
Intervention
see the composition of the test drinks in DESIGN
Study burden and risks
Burden:
During 4 days a 3-h hospital visit. Subjects are asked to come over to the
hospital in fasted state. A vein in the under arm will be canulated . Every 15
minutes, a blood sample of 5 mL will be drawn. Per test day, 14 samples will be
drawn (70 mL total). The subjects will consume 500 mL of a testdrink. During 4
evenings the subjects have to follow dietary instructions and refrain from
sports. Also the morning before vissiting the hospital, the subjects have to
refrain from sports.
Risk:
Blood sampling will be performed by experienced research nurses and will be at
low risk. No risk is expected from a single high potato protein comsumption.
Transportweg 11
9645 KZ Veendam
NL
Transportweg 11
9645 KZ Veendam
NL
Listed location countries
Age
Inclusion criteria
Age 18 - 50 years
Informed consent
Willing to consume a standardized diet on the evening before the intervention days (instructions).
Willing to refrain from sports on the evening before and on the morning of the intervention days.
Exclusion criteria
Gastrointestinal disease, such as gastric reflux, inflammatory bowel disease, pancreatic disease (self-reported)
Gastrointestinal surgery (self-reported)
Kidney disease (self-reported)
Allergy to cow's milk (self-reported)
Intensive sports (> 16 h week)
Use of protein supplements, inibitors gastric acid secretion, norit, laxatives or drugs
High alcohol intake ( > 4 consumptions/ day or > 20 consumptions per week)
Smokers
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL36997.081.11 |